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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00003954-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003954-D01, RRID:AB_10839674
- Product name
- LETM1 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human LETM1 protein.
- Antigen sequence
MASILLRSCRGRAPARLPPPPRYTVPRGSPGDPAH
LSCASTLGLRNCLNVPFGCCTPIHPVYTSSRGDHL
GCWALRPECLRIVSRAPWTSTSVGFVAVGPQCLPV
RGWHSSRPVRDDSVVEKSLKSLKDKNKKLEEGGPV
YSPPAEVVVKKSLGQRVLDELKHYYHGFRLLWIDT
KIAARMLWRILNGHSLTRRERRQFLRICADLFRLV
PFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSL
KEERLKKELRVKLELAKFLQDTIEEMALKNKAAKG
SATKDFSVFFQKIRETGERPSNEEIMRFSKLFEDE
LTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTM
RLRSIKADDKLIAEEGVDSLNVKELQAACRARGMR
ALGVTEDRLRGQLKQWLDLHLHQEIPTSLLILSRA
MYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEV
EGEQVDNKAKLEATLQEEAAIQQEHREKELQKRSE
VAKDFEPERVVAAPQRPGTEPQPEMPDTVLQSETL
KDTAPVLEGLKEEEITKEEIDILSDACSKLQEQKK
SLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGE
EKYVEESKASKRLTKRVQQMIGQIDGLISQLEMDQ
QAGKLAPANGMPTGENVISVAELINAMKQVKHIPE
SKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDV
HISTSQVAEIVATLEKEEKVEEKEKAKEKAEKEVA
EVKS- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- LETM1 MaxPab rabbit polyclonal antibody. Western Blot analysis of LETM1 expression in mouse kidney.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- LETM1 MaxPab rabbit polyclonal antibody. Western Blot analysis of LETM1 expression in A-431.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of LETM1 expression in transfected 293T cell line (H00003954-T01) by LETM1 MaxPab polyclonal antibody.Lane 1: LETM1 transfected lysate(83.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of LETM1 transfected lysate using anti-LETM1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LETM1 purified MaxPab mouse polyclonal antibody (B01P) (H00003954-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
