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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-22233 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LETM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LETM1 Polyclonal Antibody (Product # PA5-22233). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. Lane C: HeLa. Lane D: HepG2. 7.5% SDS PAGE. LETM1 Polyclonal Antibody (Product # PA5-22233) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LETM1 Polyclonal Antibody (Product # PA5-22233). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. LETM1 Polyclonal Antibody (Product # PA5-22233) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of LETM1 in methanol-fixed A431 cells using a LETM1 polyclonal antibody (Product # PA5-22233) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using LETM1 (Product # PA5-22233) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LETM1 Polyclonal Antibody detects LETM1 protein at cytoplasm on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. LETM1 Polyclonal Antibody (Product # PA5-22233) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LETM1 Polyclonal Antibody detects LETM1 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. LETM1 Polyclonal Antibody (Product # PA5-22233) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
