Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-89556 - Provider product page

- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89556, RRID:AB_11006304
- Product name
- Rabbit Polyclonal LETM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human LETM1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Endoplasmic reticulum potassium-hydrogen exchanger and small conductance calcium-activated potassium channel activities are essential for ER calcium uptake in neurons and cardiomyocytes.
Kuum M, Veksler V, Liiv J, Ventura-Clapier R, Kaasik A
Journal of cell science 2012 Feb 1;125(Pt 3):625-33
Journal of cell science 2012 Feb 1;125(Pt 3):625-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image

- Experimental details
- Simple Western: LETM1 Antibody [NBP1-89556] - Simple Western lane view shows a specific band for LETM1 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image

- Experimental details
- Simple Western: LETM1 Antibody [NBP1-89556] - Electropherogram image(s) of corresponding Simple Western lane view. LETM1 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image

- Experimental details
- Western Blot: LETM1 Antibody [NBP1-89556] - Analysis in human cell line MOLT-4.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image

- Experimental details
- Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image

- Experimental details
- Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556] - Staining of human colon shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image

- Experimental details
- Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image

- Experimental details
- Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556] - Staining in human colon and pancreas tissues using anti-LETM1 antibody. Corresponding LETM1 RNA-seq data are presented for the same tissues.