MA1-720

antibody from Invitrogen Antibodies

Targeting: GRK1 GPRK1, RHOK, RK

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA1-720 - Provider product page
Provider: Invitrogen Antibodies
Product name: GRK1 Monoclonal Antibody (G8)
Antibody type: Monoclonal
Antigen: Other
Description: MA1-720 detects G-protein-associated rhodopsin kinase 1a (GRK1a) from human, mouse, rat, bovine and chicken tissues. MA1-720 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~60 kDa protein representing GRK1a from bovine rod outer segments. Immunofluorescence staining of GRK1a in bovine retina with MA1-720 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods. The MA1-720 immunogen is full length human GRK1. This antibody recognizes an epitope in the C-terminal region of GRK1a.
Reactivity: Human, Mouse, Rat, Bovine, Chicken/Avian
Host: Mouse
Isotype: IgG
Antibody clone number: G8
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

GRK1 protein structure - MA1-720 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Rhodopsin Kinase 1a (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a Monclonal Antibody (G8) (Product # MA1-720) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Rhodopsin Kinase 1a (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a Monclonal Antibody (G8) (Product # MA1-720) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Rhodopsin Kinase 1a (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a Monclonal Antibody (G8) (Product # MA1-720) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Rhodopsin Kinase 1a was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rhodopsin Kinase 1a (G8) Mouse Monoclonal Antibody (Product # MA1-720) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Immunofluorescence analyses of the tubby mutant retina. (A) The WT tubby protein migrates at approximately 60 kDa by immunoblotting, but tubby protein expression is absent in the mutant tissues indicating that the mutation is a functional allele. The lower panel shows the actin immunoblotting results as a loading control. (B) Rhodopsin is localized primarily in photoreceptor outer segments in WT but is severely mislocalized to the cell body in the tubby mutant at one month of age. Similarly, blue and green cone opsins are normally localized to the cone outer segments in WT but are also mislocalized to the cell body and synaptic terminals in the tubby mutant retina. (C) In contrast to rhodopsin and cone opsins, peripherin/RDS and GRK, which are also integral membrane proteins, do not show mislocalization in the tubby mutant. Rod PDE and RP1 protein, which are membrane and cytoskeleton associated proteins, respectively, also manifest normal outer segment localization. IS, inner segment; ONL, outer (photoreceptor) nuclear layer; OPL, outer plexiform layer; OS, outer segment. Cell nuclei were counter stained blue by DAPI.