- MA1-721 - Invitrogen Antibodies GRK1 antibody

Submitted references Acute and Protracted Cell Death in Light-Induced Retinal Degeneration in the Canine Model of Rhodopsin Autosomal Dominant Retinitis Pigmentosa.

Sudharsan R, Simone KM, Anderson NP, Aguirre GD, Beltran WA
Investigative ophthalmology & visual science 2017 Jan 1;58(1):270-281

Phosphorylation of G protein-coupled receptor kinase 1 (GRK1) is regulated by light but independent of phototransduction in rod photoreceptors.

Osawa S, Jo R, Xiong Y, Reidel B, Tserentsoodol N, Arshavsky VY, Iuvone PM, Weiss ER
The Journal of biological chemistry 2011 Jun 10;286(23):20923-9

GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase.

Tsang SH, Woodruff ML, Chen CK, Yamashita CY, Cilluffo MC, Rao AL, Farber DB, Fain GL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Apr 26;26(17):4472-80

A short, highly active photoreceptor-specific enhancer/promoter region upstream of the human rhodopsin kinase gene.

Young JE, Vogt T, Gross KW, Khani SC
Investigative ophthalmology & visual science 2003 Sep;44(9):4076-85

GRK1-dependent phosphorylation of S and M opsins and their binding to cone arrestin during cone phototransduction in the mouse retina.

Zhu X, Brown B, Li A, Mears AJ, Swaroop A, Craft CM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Jul 9;23(14):6152-60

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Rhodopsin Kinase 1a/b (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) (Product # MA1-721) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Rhodopsin Kinase 1a/b (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) (Product # MA1-721) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Rhodopsin Kinase 1a/b (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) (Product # MA1-721) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunolocalization of GRK1a/b in bovine retina using Product # MA1-721.

MA1-721

Antibody data