36-6200

antibody from Invitrogen Antibodies

Targeting: SDC2 CD362, fibroglycan, HSPG, HSPG1, SYND2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

36-6200

Provider

Invitrogen Antibodies

Product name

Syndecan 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.25 mg/mL

Storage

-20°C

References

Submitted references

Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression.

Beauvais DM, Nelson SE, Adams KM, Stueven NA, Jung O, Rapraeger AC
The Journal of biological chemistry 2022 Jun;298(6):102029

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Heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) function as endocytic receptors for an internalizing anti-nucleic acid antibody.

Park H, Kim M, Kim HJ, Lee Y, Seo Y, Pham CD, Lee J, Byun SJ, Kwon MH
Scientific reports 2017 Oct 30;7(1):14373

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Osteoblastic heparan sulfate glycosaminoglycans control bone remodeling by regulating Wnt signaling and the crosstalk between bone surface and marrow cells.

Mansouri R, Jouan Y, Hay E, Blin-Wakkach C, Frain M, Ostertag A, Le Henaff C, Marty C, Geoffroy V, Marie PJ, Cohen-Solal M, Modrowski D
Cell death & disease 2017 Jun 29;8(6):e2902

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Syndecan-2 is a key regulator of transforming growth factor beta 2/Smad2-mediated adhesion in fibrosarcoma cells.

Mytilinaiou M, Bano A, Nikitovic D, Berdiaki A, Voudouri K, Kalogeraki A, Karamanos NK, Tzanakakis GN
IUBMB life 2013 Feb;65(2):134-43

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Differences in heparan sulfate production in cervical fibroblast cultures from women undergoing term and preterm delivery.

Akerud A, Dubicke A, Sennstrom M, Ekman-Ordeberg G, Malmstrom A
Acta obstetricia et gynecologica Scandinavica 2008;87(11):1220-8

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CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.

Chen Y, Abraham DJ, Shi-Wen X, Pearson JD, Black CM, Lyons KM, Leask A
Molecular biology of the cell 2004 Dec;15(12):5635-46

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Syndecan-2 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Syndecan-2 Rabbit polyclonal Antibody (Product # 36-6200) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing junctional localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Syndecan-2 was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Tritonª X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Syndecan-2 Rabbit Polyclonal Antibody (366200, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor¨ 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune¨ Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Syndecan-2 overexpression enriched bone surface with heparan sulfate glycosaminoglycans. ( a ) Immunohistological analysis of syndecan-2 in paraffin-embedded vertebra from 2-month-old WT ( a and b ) or ColI-Synd2 ( c and d ) mice. ( b ) Flow cytometry analysis of syndecan-2 expression. Marrow cells from 6-week-old mice were flushed from long bones and labeled with surface markers to select osteoblast precursors (CD51 + Sca-1 + cells) or osteoblasts (CD51 + Sca-1 - cells). Fluorescence intensity versus structural parameter of the cells (FSC-H) was plotted. ( c ) The median fluorescence intensity (MFI) of syndecan-2 labeling was recorded in different cell populations from WT or ColI-Synd2 mice. Data are mean+-S.E.M. ( n =7 WT and three ColI-Synd2 mice). ( d ) The MFI of heparan sulfate labeling was recorded in CD51 + Sca-1 - cells from the marrow of WT or ColI-Synd2 mice. Data are mean+-S.E.M. ( n =4 WT and four ColI-Synd2 mice). ( e ) Immunohistological analysis of heparan sulfate chains in paraffin-embedded vertebra from 2-month-old WT or ColI-Synd2 mice. The photos presented are representative views of syndecan-2 or heparan sulfate staining. Arrows indicate syndecan-2- or heparan sulfate-expressing cells. Dashed lines indicate bone surfaces. *Indicates significant difference between WT and ColI-Synd2 ( P