Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [4]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB83162-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TMIGD2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TMIGD2/CD28H in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TMIGD2/CD28H in HEK293 Human Cell Line Transfected with Human TMIGD2/CD28H and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TMIGD2/CD28H and eGFP was stained with and either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TMIGD2/CD28H in Human PBMC by Flow Cytometry. Detection of TMIGD2/CD28H in Human PBMC by Flow Cytometry.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TMIGD2/CD28H in Human PBMC by Flow Cytometry. Detection of TMIGD2/CD28H in Human PBMC by Flow Cytometry.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TMIGD2/CD28H in Human PBMC by Flow Cytometry. Detection of TMIGD2/CD28H in Human PBMC by Flow Cytometry.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- B7-H7 binding to TMIGD2/CD28H-transfected HEK293 Human Cell Line is Blocked by Human TMIGD2/CD28H Antibody. In a functional flow cytometry test, Recombinant Human B7-H7 Fc Chimera (Catalog # 156-B7, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human TMIGD2/CD28H (black dotted line). Binding is completely blocked (orange histogram) by 50 μg/mL of Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162). Mouse IgG2B Isotype Control (Catalog # MAB003) at 50 μg/mL was used as a control (blue line). Cells were stained with Mouse Anti-Human IgG1 Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A).