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- Antibody Data
- Antigen structure
- References [15]
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- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 13499-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13499-1-AP, RRID:AB_2115391
- Product name
- NGB antibody
- Antibody type
- Polyclonal
- Description
- NGB antibody (Cat. #13499-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, WB, ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Electroacupuncture improves hypoxic stress and energy metabolism to alleviate vascular cognitive impairment through activation of the HIF-1α/p53/NGB signaling pathway in rats.
Neuroglobin-promoted autophagy inhibits the proliferation, migration and invasion of human glioblastoma cells through the EGFR/PI3K/AKT/mTOR signaling pathway.
Neuroglobin regulates autophagy through mTORC1/RAPTOR/ULK-1 pathway in human neuroblastoma cells.
Neuroglobin-enriched secretome provides neuroprotection against hydrogen peroxide and mitochondrial toxin-induced cellular stress.
PTP1B Modulates Carotid Plaque Vulnerability in Atherosclerosis Through Rab5-PDGFRβ-Mediated Endocytosis Disruption and Apoptosis.
Isoquercitrin Played a Neuroprotective Role in Rats After Cerebral Ischemia/Reperfusion Through Up-Regulating Neuroglobin and Anti-Oxidative Stress.
Intravitreal Neuroglobin Mitigates Primate Experimental Glaucomatous Structural Damage in Association with Reduced Optic Nerve Microglial and Complement 3-Astrocyte Activation.
Neuroglobin expression and function in the temporal cortex of bilirubin encephalopathy rats.
Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq.
Prunus cerasoides Extract and Its Component Compounds Upregulate Neuronal Neuroglobin Levels, Mediate Antioxidant Effects, and Ameliorate Functional Losses in the Mouse Model of Cerebral Ischemia.
Hemin treatment protects neonatal rats from sevoflurane-induced neurotoxicity via the phosphoinositide 3-kinase/Akt pathway.
The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats.
Connecting the Dots in the Neuroglobin-Protein Interaction Network of an Unstressed and Ferroptotic Cell Death Neuroblastoma Model.
Neuroglobin mediates neuroprotection of hypoxic postconditioning against transient global cerebral ischemia in rats through preserving the activity of Na(+)/K(+) ATPases.
Impaired hypoxic tolerance in APP23 mice: a dysregulation of neuroprotective globin levels.
Hu P, Xu F, Zhang W, Bai L, Dai F, Ye Y, Wang J, Cheng H
Iranian journal of basic medical sciences 2026;29(1):34-42
Iranian journal of basic medical sciences 2026;29(1):34-42
Neuroglobin-promoted autophagy inhibits the proliferation, migration and invasion of human glioblastoma cells through the EGFR/PI3K/AKT/mTOR signaling pathway.
Li L, Zhang H, Li W, Yuan Z, Zhao W, Luo Z
Journal of translational medicine 2025 Nov 10;23(1):1248
Journal of translational medicine 2025 Nov 10;23(1):1248
Neuroglobin regulates autophagy through mTORC1/RAPTOR/ULK-1 pathway in human neuroblastoma cells.
Manganelli V, Costanzo M, Caissutti D, Salvatori I, Candelise N, Montalesi E, De Simone G, Ferri A, Garofalo T, Sorice M, Ruoppolo M, Longo A, Misasi R
Scientific reports 2025 Mar 4;15(1):7642
Scientific reports 2025 Mar 4;15(1):7642
Neuroglobin-enriched secretome provides neuroprotection against hydrogen peroxide and mitochondrial toxin-induced cellular stress.
Bastari G, Solar Fernandez V, Muzzi M, Moreno S, Marino M, Fiocchetti M
Cell stress 2024;8:99-111
Cell stress 2024;8:99-111
PTP1B Modulates Carotid Plaque Vulnerability in Atherosclerosis Through Rab5-PDGFRβ-Mediated Endocytosis Disruption and Apoptosis.
Zhang X, Xu R, Wang T, Li J, Sun Y, Cui S, Xing Z, Lyu X, Yang G, Jiao L, Li W
CNS neuroscience & therapeutics 2024 Nov;30(11):e70071
CNS neuroscience & therapeutics 2024 Nov;30(11):e70071
Isoquercitrin Played a Neuroprotective Role in Rats After Cerebral Ischemia/Reperfusion Through Up-Regulating Neuroglobin and Anti-Oxidative Stress.
Li X, Yi L, Liu X, Chen X, Chen S, Cai S
Transplantation proceedings 2023 Sep;55(7):1751-1761
Transplantation proceedings 2023 Sep;55(7):1751-1761
Intravitreal Neuroglobin Mitigates Primate Experimental Glaucomatous Structural Damage in Association with Reduced Optic Nerve Microglial and Complement 3-Astrocyte Activation.
Chan ASY, Tun SBB, Lynn MN, Ho C, Tun TA, Girard MJA, Sultana R, Barathi VA, Aung T, Aihara M
Biomolecules 2023 Jun 8;13(6)
Biomolecules 2023 Jun 8;13(6)
Neuroglobin expression and function in the temporal cortex of bilirubin encephalopathy rats.
Hu JH, Fan P, Zhang LR, Chen CY, Xu J, Huang J, Lu WT, Zhu SJ, Qiu GP, Xu SY, Ran JH, Gan SW
Anatomical record (Hoboken, N.J. : 2007) 2022 Feb;305(2):254-264
Anatomical record (Hoboken, N.J. : 2007) 2022 Feb;305(2):254-264
Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq.
Wang Y, Niu A, Pan Y, Cao S, Terker AS, Wang S, Fan X, Toth CL, Ramirez Solano MA, Michell DL, Contreras D, Allen RM, Zhu W, Sheng Q, Fogo AB, Vickers KC, Zhang MZ, Harris RC
Diabetes 2021 Oct;70(10):2377-2390
Diabetes 2021 Oct;70(10):2377-2390
Prunus cerasoides Extract and Its Component Compounds Upregulate Neuronal Neuroglobin Levels, Mediate Antioxidant Effects, and Ameliorate Functional Losses in the Mouse Model of Cerebral Ischemia.
Kim SD, Kim M, Wu HH, Jin BK, Jeon MS, Song YS
Antioxidants (Basel, Switzerland) 2021 Dec 30;11(1)
Antioxidants (Basel, Switzerland) 2021 Dec 30;11(1)
Hemin treatment protects neonatal rats from sevoflurane-induced neurotoxicity via the phosphoinositide 3-kinase/Akt pathway.
Yang F, Zhang Y, Tang Z, Shan Y, Wu X, Liu H
Life sciences 2020 Feb 1;242:117151
Life sciences 2020 Feb 1;242:117151
The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats.
Yang F, Shan Y, Tang Z, Wu X, Bi C, Zhang Y, Gao Y, Liu H
Frontiers in neuroscience 2019;13:537
Frontiers in neuroscience 2019;13:537
Connecting the Dots in the Neuroglobin-Protein Interaction Network of an Unstressed and Ferroptotic Cell Death Neuroblastoma Model.
Van Acker ZP, Van Raemdonck GA, Logie E, Van Acker SI, Baggerman G, Vanden Berghe W, Ponsaerts P, Dewilde S
Cells 2019 Aug 11;8(8)
Cells 2019 Aug 11;8(8)
Neuroglobin mediates neuroprotection of hypoxic postconditioning against transient global cerebral ischemia in rats through preserving the activity of Na(+)/K(+) ATPases.
Wen H, Liu L, Zhan L, Liang D, Li L, Liu D, Sun W, Xu E
Cell death & disease 2018 May 25;9(6):635
Cell death & disease 2018 May 25;9(6):635
Impaired hypoxic tolerance in APP23 mice: a dysregulation of neuroprotective globin levels.
Van Acker ZP, Luyckx E, Van Leuven W, Geuens E, De Deyn PP, Van Dam D, Dewilde S
FEBS letters 2017 May;591(10):1321-1332
FEBS letters 2017 May;591(10):1321-1332
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 13499-1-AP(NGB antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 13499-1-AP(NGB antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The NGB antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NGB. This antibody recognizes human, mouse, rat antigen. The NGB antibody has been validated for the following applications: ELISA, WB, IHC analysis.
