NBP2-59110

antibody from Novus Biologicals

Targeting: MFF C2orf33, GL004

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP2-59110

Provider

Novus Biologicals

Product name

Rabbit Polyclonal MFF Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MFF Antibody [NBP2-59110] - Detection of human and mouse MFF by western blot. Samples: Whole cell lysate from HeLa (5 and 15 ug) 293T, (50ug), and Jurkat (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MFF antibody NBP2-59110 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MFF Antibody [NBP2-59110] - Detection of human MFF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T (50ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MFF antibody NBP2-59110 used for IP at 20 ul per reaction. MFF was also immunoprecipitated by rabbit anti-MFF antibody NBP2-59109. For blotting immunoprecipitated MFF,NBP2-59110 was used at 1:250. Detection: Chemiluminescence with an exposure time of 30 seconds.