PA5-104614

antibody from Invitrogen Antibodies

Targeting: MFF C2orf33, GL004

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-104614

Provider

Invitrogen Antibodies

Product name

Phospho-C2orf33 (Ser172, Ser146) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of MFF only when phosphorylated at Ser172/146.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Autophagy-mitophagy induction attenuates cardiovascular inflammation in a murine model of Kawasaki disease vasculitis.

Marek-Iannucci S, Ozdemir AB, Moreira D, Gomez AC, Lane M, Porritt RA, Lee Y, Shimada K, Abe M, Stotland A, Zemmour D, Parker S, Sanchez-Lopez E, Van Eyk J, Gottlieb RA, Fishbein MC, Karin M, Crother TR, Rivas MN, Arditi M
JCI insight 2021 Sep 22;6(18)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 Metformin reduces cardiovascular lesions in the LCWE-dependent murine model of KD vasculitis. ( A ) Heart sections H&E staining and heart vessel inflammation score of WT mice injected with either LCWE + PBS or LCWE + Metformin (Metformin 300 mg/kg/d i.p. from day -2 to day 7), 2 weeks after LCWE injection ( n = 10/group). Scale bars: 500 uM. ( B ) Representative pictures of the abdominal aorta, maximal aorta diameter, and abdominal aorta area measurements from LCWE + PBS or LCWE + Metformin treated mice ( n = 10/group), 2 weeks after LCWE injection. ( C ) Representative pictures of FLICA staining and quantification in heart tissue sections of LCWE + PBS- or LCWE + Metformin-treated WT mice, 1 week after LCWE injection ( n = 5/group). White arrows indicate FLICA + cells. Scale bars: 100 um. ( D ) IL-1beta levels in the serum of WT mice injected with either LCWE + PBS or LCWE + Metformin, at 1 week after LCWE injection ( n = 8-9/group). ( E and F ) Western blot analysis ( E ) and quantification ( F ) of complex I (CI), CII, CIII, CIV, and CV reflecting mitochondrial content, in whole lysates of heart tissues collected from LCWE + PBS and LCWE + Metformin, 1 week after LCWE injection ( n = 4/group). ( G ) Representative DHE staining and ROS quantification in abdominal aorta tissue cross-sections collected from LCWE + PBS- or LCWE + Metformin-treated mice, 1-week after injection ( n = 7-9/group). Scale bar: 100 um. ( H and I ) Representative Western blot analysis images