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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [33]
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- Immunocytochemistry [3]
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- Product number
- ADI-SPA-827-D - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-SPA-827-D, RRID:AB_2039327
- Product name
- KDEL, mAb (10C3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Protein G affinity purified. Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
- Isotype
- IgG
- Antibody clone number
- 10C3
Submitted references Cellular localization and tissue distribution of endogenous DFCP1 protein.
Pancreas-Specific Sirt1-Deficiency in Mice Compromises Beta-Cell Function without Development of Hyperglycemia.
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP.
Endogenously tagged rab proteins: a resource to study membrane trafficking in Drosophila.
PINK1 and Parkin control localized translation of respiratory chain component mRNAs on mitochondria outer membrane.
A53T human α-synuclein overexpression in transgenic mice induces pervasive mitochondria macroautophagy defects preceding dopamine neuron degeneration.
Endoplasmic Reticulum (ER) Stress Induces Sirtuin 1 (SIRT1) Expression via the PI3K-Akt-GSK3β Signaling Pathway and Promotes Hepatocellular Injury.
CHOP deficiency prevents methylglyoxal-induced myocyte apoptosis and cardiac dysfunction.
GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses.
A novel UGGT1 and p97-dependent checkpoint for native ectodomains with ionizable intramembrane residue.
Activation of the proapoptotic unfolded protein response in plaques of the human carotid artery.
Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase.
Alteration of endoplasmic reticulum lipid rafts contributes to lipotoxicity in pancreatic β-cells.
Nedd4 family-interacting protein 1 (Ndfip1) is required for the exosomal secretion of Nedd4 family proteins.
The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated degradation pathway.
Endoplasmic reticulum stress induces leptin resistance.
Pofut1 is required for the proper localization of the Notch receptor during mouse development.
Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases.
Tissue and subcellular distribution of CLIC1.
The amyloid precursor protein protects PC12 cells against endoplasmic reticulum stress-induced apoptosis.
Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity.
Boca, an endoplasmic reticulum protein required for wingless signaling and trafficking of LDL receptor family members in Drosophila.
Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae.
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum.
A protein sequence that can encode native structure by disfavoring alternate conformations.
Developmental regulation of neuronal K(Ca) channels by TGFbeta1: an essential role for PI3 kinase signaling and membrane insertion.
Efficacy of plant-produced recombinant antibodies against HCG.
Mass transport of proform of a KDEL-tailed cysteine proteinase (SH-EP) to protein storage vacuoles by endoplasmic reticulum-derived vesicle is involved in protein mobilization in germinating seeds.
Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins.
Mutants of the yeast Yarrowia lipolytica defective in protein exit from the endoplasmic reticulum are also defective in peroxisome biogenesis.
Identification of amino acids in the binding pocket of the human KDEL receptor.
Identification by anti-idiotype antibodies of an intracellular membrane protein that recognizes a mammalian endoplasmic reticulum retention signal.
The retention signal for soluble proteins of the endoplasmic reticulum.
Nanao T, Koike M, Yamaguchi J, Sasaki M, Uchiyama Y
Biomedical research (Tokyo, Japan) 2015;36(2):121-33
Biomedical research (Tokyo, Japan) 2015;36(2):121-33
Pancreas-Specific Sirt1-Deficiency in Mice Compromises Beta-Cell Function without Development of Hyperglycemia.
Pinho AV, Bensellam M, Wauters E, Rees M, Giry-Laterriere M, Mawson A, Ly le Q, Biankin AV, Wu J, Laybutt DR, Rooman I
PloS one 2015;10(6):e0128012
PloS one 2015;10(6):e0128012
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP.
Teng Y, Rezvani K, De Biasi M
Biochemical pharmacology 2015 Oct 15;97(4):518-30
Biochemical pharmacology 2015 Oct 15;97(4):518-30
Endogenously tagged rab proteins: a resource to study membrane trafficking in Drosophila.
Dunst S, Kazimiers T, von Zadow F, Jambor H, Sagner A, Brankatschk B, Mahmoud A, Spannl S, Tomancak P, Eaton S, Brankatschk M
Developmental cell 2015 May 4;33(3):351-65
Developmental cell 2015 May 4;33(3):351-65
PINK1 and Parkin control localized translation of respiratory chain component mRNAs on mitochondria outer membrane.
Gehrke S, Wu Z, Klinkenberg M, Sun Y, Auburger G, Guo S, Lu B
Cell metabolism 2015 Jan 6;21(1):95-108
Cell metabolism 2015 Jan 6;21(1):95-108
A53T human α-synuclein overexpression in transgenic mice induces pervasive mitochondria macroautophagy defects preceding dopamine neuron degeneration.
Chen L, Xie Z, Turkson S, Zhuang X
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Jan 21;35(3):890-905
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Jan 21;35(3):890-905
Endoplasmic Reticulum (ER) Stress Induces Sirtuin 1 (SIRT1) Expression via the PI3K-Akt-GSK3β Signaling Pathway and Promotes Hepatocellular Injury.
Koga T, Suico MA, Shimasaki S, Watanabe E, Kai Y, Koyama K, Omachi K, Morino-Koga S, Sato T, Shuto T, Mori K, Hino S, Nakao M, Kai H
The Journal of biological chemistry 2015 Dec 18;290(51):30366-74
The Journal of biological chemistry 2015 Dec 18;290(51):30366-74
CHOP deficiency prevents methylglyoxal-induced myocyte apoptosis and cardiac dysfunction.
Nam DH, Han JH, Lee TJ, Shishido T, Lim JH, Kim GY, Woo CH
Journal of molecular and cellular cardiology 2015 Aug;85:168-77
Journal of molecular and cellular cardiology 2015 Aug;85:168-77
GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses.
Hotokezaka Y, Katayama I, van Leyen K, Nakamura T
Cell death & disease 2015 Apr 16;6:e1719
Cell death & disease 2015 Apr 16;6:e1719
A novel UGGT1 and p97-dependent checkpoint for native ectodomains with ionizable intramembrane residue.
Merulla J, Soldà T, Molinari M
Molecular biology of the cell 2015 Apr 15;26(8):1532-42
Molecular biology of the cell 2015 Apr 15;26(8):1532-42
Activation of the proapoptotic unfolded protein response in plaques of the human carotid artery.
Dorweiler B, Grechowa I, Wallrath A, Vahl CF, Horke S
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2014 Sep;48(3):248-57
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2014 Sep;48(3):248-57
Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase.
Takayanagi T, Crawford KJ, Kobayashi T, Obama T, Tsuji T, Elliott KJ, Hashimoto T, Rizzo V, Eguchi S
Clinical science (London, England : 1979) 2014 Jun;126(11):785-94
Clinical science (London, England : 1979) 2014 Jun;126(11):785-94
Alteration of endoplasmic reticulum lipid rafts contributes to lipotoxicity in pancreatic β-cells.
Boslem E, Weir JM, MacIntosh G, Sue N, Cantley J, Meikle PJ, Biden TJ
The Journal of biological chemistry 2013 Sep 13;288(37):26569-82
The Journal of biological chemistry 2013 Sep 13;288(37):26569-82
Nedd4 family-interacting protein 1 (Ndfip1) is required for the exosomal secretion of Nedd4 family proteins.
Putz U, Howitt J, Lackovic J, Foot N, Kumar S, Silke J, Tan SS
The Journal of biological chemistry 2008 Nov 21;283(47):32621-7
The Journal of biological chemistry 2008 Nov 21;283(47):32621-7
The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated degradation pathway.
Duriez M, Rossignol JM, Sitterlin D
The Journal of biological chemistry 2008 Nov 21;283(47):32352-60
The Journal of biological chemistry 2008 Nov 21;283(47):32352-60
Endoplasmic reticulum stress induces leptin resistance.
Hosoi T, Sasaki M, Miyahara T, Hashimoto C, Matsuo S, Yoshii M, Ozawa K
Molecular pharmacology 2008 Dec;74(6):1610-9
Molecular pharmacology 2008 Dec;74(6):1610-9
Pofut1 is required for the proper localization of the Notch receptor during mouse development.
Okamura Y, Saga Y
Mechanisms of development 2008 Aug;125(8):663-73
Mechanisms of development 2008 Aug;125(8):663-73
Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases.
Ito D, Suzuki N
Annals of neurology 2007 Mar;61(3):237-50
Annals of neurology 2007 Mar;61(3):237-50
Tissue and subcellular distribution of CLIC1.
Ulmasov B, Bruno J, Woost PG, Edwards JC
BMC cell biology 2007 Feb 27;8:8
BMC cell biology 2007 Feb 27;8:8
The amyloid precursor protein protects PC12 cells against endoplasmic reticulum stress-induced apoptosis.
Kögel D, Schomburg R, Schürmann T, Reimertz C, König HG, Poppe M, Eckert A, Müller WE, Prehn JH
Journal of neurochemistry 2003 Oct;87(1):248-56
Journal of neurochemistry 2003 Oct;87(1):248-56
Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity.
Watson LM, Chan AK, Berry LR, Li J, Sood SK, Dickhout JG, Xu L, Werstuck GH, Bajzar L, Klamut HJ, Austin RC
The Journal of biological chemistry 2003 May 9;278(19):17438-47
The Journal of biological chemistry 2003 May 9;278(19):17438-47
Boca, an endoplasmic reticulum protein required for wingless signaling and trafficking of LDL receptor family members in Drosophila.
Culi J, Mann RS
Cell 2003 Feb 7;112(3):343-54
Cell 2003 Feb 7;112(3):343-54
Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae.
Kauffman KJ, Pridgen EM, Doyle FJ 3rd, Dhurjati PS, Robinson AS
Biotechnology progress 2002 Sep-Oct;18(5):942-50
Biotechnology progress 2002 Sep-Oct;18(5):942-50
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum.
Serwold T, Gonzalez F, Kim J, Jacob R, Shastri N
Nature 2002 Oct 3;419(6906):480-3
Nature 2002 Oct 3;419(6906):480-3
A protein sequence that can encode native structure by disfavoring alternate conformations.
Wigley WC, Corboy MJ, Cutler TD, Thibodeau PH, Oldan J, Lee MG, Rizo J, Hunt JF, Thomas PJ
Nature structural biology 2002 May;9(5):381-8
Nature structural biology 2002 May;9(5):381-8
Developmental regulation of neuronal K(Ca) channels by TGFbeta1: an essential role for PI3 kinase signaling and membrane insertion.
Lhuillier L, Dryer SE
Journal of neurophysiology 2002 Aug;88(2):954-64
Journal of neurophysiology 2002 Aug;88(2):954-64
Efficacy of plant-produced recombinant antibodies against HCG.
Kathuria S, Sriraman R, Nath R, Sack M, Pal R, Artsaenko O, Talwar GP, Fischer R, Finnern R
Human reproduction (Oxford, England) 2002 Aug;17(8):2054-61
Human reproduction (Oxford, England) 2002 Aug;17(8):2054-61
Mass transport of proform of a KDEL-tailed cysteine proteinase (SH-EP) to protein storage vacuoles by endoplasmic reticulum-derived vesicle is involved in protein mobilization in germinating seeds.
Toyooka K, Okamoto T, Minamikawa T
The Journal of cell biology 2000 Feb 7;148(3):453-64
The Journal of cell biology 2000 Feb 7;148(3):453-64
Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins.
Choukhi A, Ung S, Wychowski C, Dubuisson J
Journal of virology 1998 May;72(5):3851-8
Journal of virology 1998 May;72(5):3851-8
Mutants of the yeast Yarrowia lipolytica defective in protein exit from the endoplasmic reticulum are also defective in peroxisome biogenesis.
Titorenko VI, Rachubinski RA
Molecular and cellular biology 1998 May;18(5):2789-803
Molecular and cellular biology 1998 May;18(5):2789-803
Identification of amino acids in the binding pocket of the human KDEL receptor.
Scheel AA, Pelham HR
The Journal of biological chemistry 1998 Jan 23;273(4):2467-72
The Journal of biological chemistry 1998 Jan 23;273(4):2467-72
Identification by anti-idiotype antibodies of an intracellular membrane protein that recognizes a mammalian endoplasmic reticulum retention signal.
Vaux D, Tooze J, Fuller S
Nature 1990 Jun 7;345(6275):495-502
Nature 1990 Jun 7;345(6275):495-502
The retention signal for soluble proteins of the endoplasmic reticulum.
Pelham HR
Trends in biochemical sciences 1990 Dec;15(12):483-6
Trends in biochemical sciences 1990 Dec;15(12):483-6
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis: Lane 1: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked) (Prod No. ADI-LYC-HL101).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10µg/ml.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).
