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- Immunocytochemistry [1]
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- Product number
- PA5-100892 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP7A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total Cytochrome P450 7A1.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Rosmarinic Acid Exhibits a Lipid-Lowering Effect by Modulating the Expression of Reverse Cholesterol Transporters and Lipid Metabolism in High-Fat Diet-Fed Mice.
Nyandwi JB, Ko YS, Jin H, Yun SP, Park SW, Kim HJ
Biomolecules 2021 Oct 6;11(10)
Biomolecules 2021 Oct 6;11(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CYP7A1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CYP7A1 polyclonal antibody (Product # PA5-100892) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP7A1 in mouse liver tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CYP7A1 polyclonal antibody (Product # PA5-100892) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 RA significantly induces ABCG5/8 and ABCA1 protein levels, which are involved in hepatobilial cholesterol excretion, and CYP7A1 protein levels, a key rate-limiting enzyme for bile acid biosynthesis in HFD-fed mice. ( A - D ) ABCG5, ABCG8, ABCA1, and ( E , F ) CYP7A1 protein levels were examined in liver tissues by western blot analysis, and the relative protein levels are presented as the mean +- SD ( n = 4-6). ** p < 0.01 vs. ND control mice; # p < 0.05, ## p < 0.01, vs. HFD mice; $ p < 0.05, $$ p < 0.01 vs. RA 50.
