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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ5717 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CYP7A1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific CYP7A1 antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/Immunohistochemistry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the CYP7A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) mouse liver, 3) monkey liver, 4) human HepG2 and 5) human HeLa lysate with CYP7A1 antibody. Predicted molecular weight ~54 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with CYP7A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CYP7A1 antibody.
