AF6705

antibody from R&D Systems

Targeting: ALDH3A1 ALDH3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6705

Provider

R&D Systems

Product name

Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Aldehyde Dehydrogenase 3-A1/ALDH3A1 in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P30838


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 by Western Blot. Western blot shows lysates of human stomach tissue, human lung tissue, human liver tissue, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldehyde Dehydrogenase 3-A1/ALDH3A1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Aldehyde Dehydrogenase 3-A1/ALDH3A1 in A549 Human Cell Line. Aldehyde Dehydrogenase 3-A1/ALDH3A1 was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Aldehyde Dehydrogenase 3-A1/ALDH3A1 in Human Normal Lung and Lung Cancer Tissue. Aldehyde Dehydrogenase 3-A1/ALDH3A1 was detected in immersion fixed paraffin-embedded sections of human normal lung and lung cancer tissue using Sheep Anti-Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.