Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- AM20310PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20310PU-N, RRID:AB_10756395
- Product name
- anti Bax inhibitor 1
- Antibody type
- Monoclonal
- Antigen
- A mixture of three synthetic peptides corresponding to the N-terminal, internal and C-terminal regions of Human Bi-1.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 20F565
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Bi-1 with AM20310PU-N, clone 20F565. Lane 1: in vitro translated human Bi-1 protein. Lanes 2 and 3: Immunoprecioitation/Western Blot of Bi-1 protein enriched in the transgenic mouse brain (Lane 2) or wildtype brain (Lane 3). Immunoprecioitation was done with a polyclonal Bi-1 antibody, followed by Western Blot with AM20310PU-N
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis using Bi-1 antibody (Cat.-No.: AM20310PU-N) at 0.5 µg/ml: <b>A</b> overview of stained human and mouse TMA, <b>B-C</b> immunoreactivity in human archicortex (positive neurons of hippocampal gyrus) at 200X and 400X, <b>D-E</b> strong immunoreactivity in neurons of human dorsal root ganglion.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human testis stained with Bi-1 antibody (Cat.-No.: AM20310PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.