Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA1-41678 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bi-1 Monoclonal Antibody (20F248)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA1-41678 is derived from a mixture of three synthetic peptides corresponding to the N-terminal, internal and C-terminal regions of human B1. Epitope mapping indicates that the antibody recognizes an epitope in the N-terminal region of Bi-1.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- 20F248
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bi-1 in A) A549 and B) human lung lysate. Samples were incubated in Bi-1 monoclonal antibody (Product # MA1-41678) using a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Bax inhibitor 1 in HeLa cells. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti Bax inhibitor 1 (20F248) Antibody (Product # MA1-41678) at a 1:200 dilution overnight at 4C and detected with an anti-mouse IgM Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin (Red) at a 1:200 dilution. Nuclei wre counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Bi-1 in THP-1 cells. Samples were incubated in Bi-1 monoclonal antibody (Product # MA1-41678) using a dilution of 1.0 µg/mL for 30 minutes at room temperature followed by Dylight488-conjugated anti-mouse secondary antibody. Antibody (blue) and a matched isotype control NBP2-27284 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.