MA5-16225

antibody from Invitrogen Antibodies

Targeting: TMBIM6 BAXI1, BI-1, TEGT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-16225

Provider

Invitrogen Antibodies

Product name

Bi-1 Monoclonal Antibody (20F565)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

20F565

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Bi-1 using a Bi-1 monoclonal antibody (Product # MA5-16225). Lane 1: in vitro translated human Bi-1 protein. Lanes 2 and 3: IP of Bi-1 protein enriched in the transgenic mouse brain (Lane 2) or wildtype brain (Lane 3), followed by Western blot.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Bi-1 in in vitro translated human Bi-1 protein, Bi-1 protein enriched in the transgenic mouse brain, wildtype brain. Samples were incubated in Bi-1 monoclonal antibody (Product # MA5-16225). Lane 1: in vitro translated human Bi-1 protein. Lanes 2 and 3: IP/WB of Bi-1 protein enriched in the transgenic mouse brain (Lane 2) or wildtype brain (Lane 3). IP was done with a polyclonal Bi-1 antibody, followed by WB with this antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Bi-1 in formalin-fixed paraffin-embedded tissue section of human liver cancer. Samples were incubated in Bi-1 monoclonal antibody (Product # MA5-16225) using a dilution of 5 µg/mL. Cytoplasmic immuno-positivity of Bax inhibitor 1 (TMBIM6) was observed in the hepatis cancer cells whereas the cells of tumor stroma did not develop any staining [10X Magnification].

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Bi-1 in human and mouse TMA, human archicortex, neurons of human dorsal root ganglion. Samples were incubated with Bi-1 monoclonal antibody (Product # MA5-16225) using a dilution of 5 µg/mL. A) overview of stained human and mouse TMA, B-C) immunoreactivity in human archicortex (positive neurons of hippocampal gyrus) at 200X and 400X, D-E) strong immunoreactivity in neurons of human dorsal root ganglion.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Bi-1 in formalin-fixed, paraffin-embedded human brain. Samples were incubated in Bi-1 monoclonal antibody (Product # MA5-16225) using a dilution of 0.5 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.