Antibody data
- Antibody Data
- Antigen structure
- References [33]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 14498-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14498-1-AP, RRID:AB_2076918
- Product name
- CEP250/CNAP1 antibody
- Antibody type
- Polyclonal
- Description
- CEP250/CNAP1 antibody (Cat. #14498-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Interactome Analysis Reveals a Link of the Novel ALMS1-CEP70 Complex to Centrosomal Clusters.
Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.
The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation.
BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms.
The phosphorylation of PHF5A by TrkA-ERK1/2-ABL1 cascade regulates centrosome separation.
cNap1 bridges centriole contact sites to maintain centrosome cohesion.
The mechanism of acentrosomal spindle assembly in human oocytes.
Postmitotic centriole disengagement and maturation leads to centrosome amplification in polyploid trophoblast giant cells.
Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement.
Centrosome linker protein C-Nap1 maintains stem cells in mouse testes.
Arf-like Protein 2 (ARL2) Controls Microtubule Neogenesis during Early Postnatal Photoreceptor Development.
Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells.
OCRL regulates lysosome positioning and mTORC1 activity through SSX2IP-mediated microtubule anchoring.
The Small-Molecule Inhibitor MRIA9 Reveals Novel Insights into the Cell Cycle Roles of SIK2 in Ovarian Cancer Cells.
Aurora kinase A is essential for meiosis in mouse oocytes.
RACK1 regulates centriole duplication through promoting the activation of polo-like kinase 1 by Aurora A.
hVFL3/CCDC61 is a component of mother centriole subdistal appendages required for centrosome cohesion and positioning.
Bisphenol A disrupts mitotic progression via disturbing spindle attachment to kinetochore and centriole duplication in cancer cell lines.
Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes.
A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals.
Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.
Separation and Loss of Centrioles From Primordidal Germ Cells To Mature Oocytes In The Mouse.
Stable centrosomal roots disentangle to allow interphase centriole independence.
Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.
Novel Candidate Genes and a Wide Spectrum of Structural and Point Mutations Responsible for Inherited Retinal Dystrophies Revealed by Exome Sequencing.
Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins.
Chronic Exposure to Particulate Chromate Induces Premature Centrosome Separation and Centriole Disengagement in Human Lung Cells.
The tumor suppressor proteins ASPP1 and ASPP2 interact with C-Nap1 and regulate centrosome linker reassembly.
TEIF associated centrosome activity is regulated by EGF/PI3K/Akt signaling.
Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion.
Woerz F, Hoffmann F, Antony S, Bolz S, Jarboui MA, Junger K, Klose F, Stehle IF, Boldt K, Ueffing M, Beyer T
Molecular & cellular proteomics : MCP 2024 Jan;23(1):100701
Molecular & cellular proteomics : MCP 2024 Jan;23(1):100701
Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.
Song H, Kim EH, Hong J, Gwon D, Kim JW, Bae GU, Jang CY
Cell death and differentiation 2023 Sep;30(9):2151-2166
Cell death and differentiation 2023 Sep;30(9):2151-2166
The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation.
Wang Y, Risteski P, Yang Y, Chen H, Droby G, Walens A, Jayaprakash D, Troester M, Herring L, Chernoff J, Tolić IM, Bowser J, Vaziri C
Nucleic acids research 2023 Oct 27;51(19):10568-10589
Nucleic acids research 2023 Oct 27;51(19):10568-10589
BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms.
Singh A, Busacca S, Gaba A, Sheaff M, Poile C, Nakas A, Dzialo J, Bzura A, Dawson AG, Fennell DA, Fry AM
Oncogene 2023 Feb;42(8):572-585
Oncogene 2023 Feb;42(8):572-585
The phosphorylation of PHF5A by TrkA-ERK1/2-ABL1 cascade regulates centrosome separation.
Song C, Zhang Y, Li Y, Bie J, Wang Z, Yang X, Li H, Zhu L, Zhang T, Chang Q, Luo J
Cell death & disease 2023 Feb 9;14(2):98
Cell death & disease 2023 Feb 9;14(2):98
cNap1 bridges centriole contact sites to maintain centrosome cohesion.
Mahen R
PLoS biology 2022 Oct;20(10):e3001854
PLoS biology 2022 Oct;20(10):e3001854
The mechanism of acentrosomal spindle assembly in human oocytes.
Wu T, Dong J, Fu J, Kuang Y, Chen B, Gu H, Luo Y, Gu R, Zhang M, Li W, Dong X, Sun X, Sang Q, Wang L
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361
Postmitotic centriole disengagement and maturation leads to centrosome amplification in polyploid trophoblast giant cells.
Buss G, Stratton MB, Milenkovic L, Stearns T
Molecular biology of the cell 2022 Nov 1;33(13):ar118
Molecular biology of the cell 2022 Nov 1;33(13):ar118
Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement.
Fdez E, Madero-Pérez J, Lara Ordóñez AJ, Naaldijk Y, Fasiczka R, Aiastui A, Ruiz-Martínez J, López de Munain A, Cowley SA, Wade-Martins R, Hilfiker S
iScience 2022 Jun 17;25(6):104476
iScience 2022 Jun 17;25(6):104476
Centrosome linker protein C-Nap1 maintains stem cells in mouse testes.
Dang H, Martin-Villalba A, Schiebel E
EMBO reports 2022 Jul 5;23(7):e53805
EMBO reports 2022 Jul 5;23(7):e53805
Arf-like Protein 2 (ARL2) Controls Microtubule Neogenesis during Early Postnatal Photoreceptor Development.
Gerstner CD, Reed M, Dahl TM, Ying G, Frederick JM, Baehr W
Cells 2022 Dec 30;12(1)
Cells 2022 Dec 30;12(1)
Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells.
Chu Z, Gruss OJ
Cells 2022 Apr 1;11(7)
Cells 2022 Apr 1;11(7)
OCRL regulates lysosome positioning and mTORC1 activity through SSX2IP-mediated microtubule anchoring.
Wang B, He W, Prosseda PP, Li L, Kowal TJ, Alvarado JA, Wang Q, Hu Y, Sun Y
EMBO reports 2021 Jul 5;22(7):e52173
EMBO reports 2021 Jul 5;22(7):e52173
The Small-Molecule Inhibitor MRIA9 Reveals Novel Insights into the Cell Cycle Roles of SIK2 in Ovarian Cancer Cells.
Raab M, Rak M, Tesch R, Gasimli K, Becker S, Knapp S, Strebhardt K, Sanhaji M
Cancers 2021 Jul 21;13(15)
Cancers 2021 Jul 21;13(15)
Aurora kinase A is essential for meiosis in mouse oocytes.
Blengini CS, Ibrahimian P, Vaskovicova M, Drutovic D, Solc P, Schindler K
PLoS genetics 2021 Apr;17(4):e1009327
PLoS genetics 2021 Apr;17(4):e1009327
RACK1 regulates centriole duplication through promoting the activation of polo-like kinase 1 by Aurora A.
Yoshino Y, Kobayashi A, Qi H, Endo S, Fang Z, Shindo K, Kanazawa R, Chiba N
Journal of cell science 2020 Sep 1;133(17)
Journal of cell science 2020 Sep 1;133(17)
hVFL3/CCDC61 is a component of mother centriole subdistal appendages required for centrosome cohesion and positioning.
Pizon V, Gaudin N, Poteau M, Cifuentes-Diaz C, Demdou R, Heyer V, Reina San Martin B, Azimzadeh J
Biology of the cell 2020 Jan;112(1):22-37
Biology of the cell 2020 Jan;112(1):22-37
Bisphenol A disrupts mitotic progression via disturbing spindle attachment to kinetochore and centriole duplication in cancer cell lines.
Kim S, Gwon D, Kim JA, Choi H, Jang CY
Toxicology in vitro : an international journal published in association with BIBRA 2019 Sep;59:115-125
Toxicology in vitro : an international journal published in association with BIBRA 2019 Sep;59:115-125
Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes.
Busselez J, Chichón FJ, Rodríguez MJ, Alpízar A, Gharbi SI, Franch M, Melero R, Paradela A, Carrascosa JL, Carazo JM
Scientific reports 2019 May 10;9(1):7187
Scientific reports 2019 May 10;9(1):7187
A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
So C, Seres KB, Steyer AM, Mönnich E, Clift D, Pejkovska A, Möbius W, Schuh M
Science (New York, N.Y.) 2019 Jun 28;364(6447)
Science (New York, N.Y.) 2019 Jun 28;364(6447)
c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Gwon D, Hong J, Jang CY
Molecules and cells 2019 Dec 31;42(12):840-849
Molecules and cells 2019 Dec 31;42(12):840-849
Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals.
Yaguchi K, Yamamoto T, Matsui R, Tsukada Y, Shibanuma A, Kamimura K, Koda T, Uehara R
The Journal of cell biology 2018 Jul 2;217(7):2463-2483
The Journal of cell biology 2018 Jul 2;217(7):2463-2483
Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
Tu HQ, Qin XH, Liu ZB, Song ZQ, Hu HB, Zhang YC, Chang Y, Wu M, Huang Y, Bai YF, Wang G, Han QY, Li AL, Zhou T, Liu F, Zhang XM, Li HY
Nature communications 2018 Dec 11;9(1):5277
Nature communications 2018 Dec 11;9(1):5277
PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.
Sydor AM, Coyaud E, Rovelli C, Laurent E, Liu H, Raught B, Mennella V
eLife 2018 Aug 31;7
eLife 2018 Aug 31;7
Separation and Loss of Centrioles From Primordidal Germ Cells To Mature Oocytes In The Mouse.
Simerly C, Manil-Ségalen M, Castro C, Hartnett C, Kong D, Verlhac MH, Loncarek J, Schatten G
Scientific reports 2018 Aug 24;8(1):12791
Scientific reports 2018 Aug 24;8(1):12791
Stable centrosomal roots disentangle to allow interphase centriole independence.
Mahen R
PLoS biology 2018 Apr;16(4):e2003998
PLoS biology 2018 Apr;16(4):e2003998
Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.
Mori M, Hazan R, Danielian PS, Mahoney JE, Li H, Lu J, Miller ES, Zhu X, Lees JA, Cardoso WV
Nature communications 2017 Jul 4;8:15857
Nature communications 2017 Jul 4;8:15857
Novel Candidate Genes and a Wide Spectrum of Structural and Point Mutations Responsible for Inherited Retinal Dystrophies Revealed by Exome Sequencing.
de Castro-Miró M, Tonda R, Escudero-Ferruz P, Andrés R, Mayor-Lorenzo A, Castro J, Ciccioli M, Hidalgo DA, Rodríguez-Ezcurra JJ, Farrando J, Pérez-Santonja JJ, Cormand B, Marfany G, Gonzàlez-Duarte R
PloS one 2016;11(12):e0168966
PloS one 2016;11(12):e0168966
Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins.
Cervenka I, Valnohova J, Bernatik O, Harnos J, Radsetoulal M, Sedova K, Hanakova K, Potesil D, Sedlackova M, Salasova A, Steinhart Z, Angers S, Schulte G, Hampl A, Zdrahal Z, Bryja V
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 16;113(33):9304-9
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 16;113(33):9304-9
Chronic Exposure to Particulate Chromate Induces Premature Centrosome Separation and Centriole Disengagement in Human Lung Cells.
Martino J, Holmes AL, Xie H, Wise SS, Wise JP Sr
Toxicological sciences : an official journal of the Society of Toxicology 2015 Oct;147(2):490-9
Toxicological sciences : an official journal of the Society of Toxicology 2015 Oct;147(2):490-9
The tumor suppressor proteins ASPP1 and ASPP2 interact with C-Nap1 and regulate centrosome linker reassembly.
Zhang Y, Wang Y, Wei Y, Ma J, Peng J, Wumaier R, Shen S, Zhang P, Yu L
Biochemical and biophysical research communications 2015 Mar 13;458(3):494-500
Biochemical and biophysical research communications 2015 Mar 13;458(3):494-500
TEIF associated centrosome activity is regulated by EGF/PI3K/Akt signaling.
Zhao J, Zou Y, Liu H, Wang H, Zhang H, Hou W, Li X, Jia X, Zhang J, Hou L, Zhang B
Biochimica et biophysica acta 2014 Sep;1843(9):1851-64
Biochimica et biophysica acta 2014 Sep;1843(9):1851-64
Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion.
Fang G, Zhang D, Yin H, Zheng L, Bi X, Yuan L
Journal of cell science 2014 Apr 15;127(Pt 8):1631-9
Journal of cell science 2014 Apr 15;127(Pt 8):1631-9
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14498-1-AP(CEP250,C-NAP1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CEP250 antibody 14498-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human heart using 14498-1-AP(CEP250,C-NAP1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human heart using 14498-1-AP(CEP250,C-NAP1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue