14498-1-AP

antibody from Invitrogen Antibodies

Targeting: CEP250 C-NAP1, CEP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14498-1-AP

Provider

Invitrogen Antibodies

Product name

CEP250/CNAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: RKLKNSQEA QQRQATLVRK LQAKVLQYRS WCQELEKRLE ATGGPIPQRW ENVEEPNLDE LLVRLEEEQQ RCESLAEVNT QLRLHMEKAD VVNKALREDV EKLTVDWSRA RDELMRKESQ WQMEQEFFKG YLKGEHGRLL SLWREVVTFR RHFLEMKSAT DRDLMELKAE HVRLSGSLLT CCLRLTVGAQ SREPNGSGRM DGREPAQLLL LLAKTQELEK EAHERSQELI QLKSQGDLEK AELQDRVTEL SALLT (40-293 aa encoded by B C001433)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.46 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CEP250 antibody 14498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 14498-1-AP ( CEP250/CNAP1 antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 14498-1-AP ( CEP250/CNAP1 antibody at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CEP250/CNAP1 (IP:14498-1-AP, 5ug; Detection:14498-1-AP 1:500) with HeLa cells lysate 1200ug.