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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- PA5-29320 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MST2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, THP-1, NCI-H929, 293T mock, 293T transfected STK3. Predicted reactivity: Mouse (91%), Rat (93%), Xenopus laevis (86%), Chicken (94%), Rhesus Monkey (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MST2 Polyclonal Antibody (Product # PA5-29320). Sample (30 µg of whole cell lysate). Lane A: THP-1. Lane B: NCI-H929 . 7.5% SDS PAGE. MST2 Polyclonal Antibody (Product # PA5-29320) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MST2 Polyclonal Antibody detects STK3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MST2 Polyclonal Antibody (Product # PA5-29320) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MST2 Polyclonal Antibody (Product # PA5-29320). Sample (30 µg of whole cell lysate). Lane A: Non-transfected 293T lysates. Lane B: STK3 transfected 293T lysates . 7.5% SDS PAGE. MST2 Polyclonal Antibody (Product # PA5-29320) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MST2 Polyclonal Antibody immunoprecipitates STK3 protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of STK3 protein by 4 µg MST2 Polyclonal Antibody (Product # PA5-29320). 7.5 % SDS-PAGE. The immunoprecipitated STK3 protein was detected by MST2 Polyclonal Antibody (Product # PA5-29320) diluted at 1:500.
