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- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-72863 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAM29 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell carcinoma.
Li SL, Jiang TQ, Cao QW, Liu SM
Journal of chemotherapy (Florence, Italy) 2021 Feb;33(1):40-50
Journal of chemotherapy (Florence, Italy) 2021 Feb;33(1):40-50
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of ADAM29 in human testis tissue with ADAM29 Polyclonal Antibody (Product # PA5-72863) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of ADAM29 in human testis tissue with ADAM29 Polyclonal Antibody (Product # PA5-72863) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Detection of knockdown and overexpression of ADAM29 efficiency. (a and b) QRT-PCR was applied to measure the mRNA level of ADAM29 in Caki-1 (a) and 786-O (b) cells with different treatment. (c and d) Western blot was applied to detect the protein level of ADAM29 in Caki-1 (c) and 786-O (d) cells with different treatment. Caki-1 cells were transfected with si-ADAM29#1, si-ADAM29#2, si-con. 786-O cells were transfected with pcDNA3.1-ADAM29 and vector. The control group received non-treatment. ** p < 0.01 vs. si-con group, vector group or con group.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Effects of knockdown or overexpression of ADAM29 on proliferation- and motion-related proteins. Western blot assay was adopted to determine the effect of ADAM29 knockdown (a) or overexpression (b) on the level of Cyclin D1, PCNA, MMP9 and Snail. ** p < 0.01 vs. si-con group or vector group.
