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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 13915-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13915-1-AP, RRID:AB_2194804
- Product name
- SPAG8 antibody
- Antibody type
- Polyclonal
- Description
- SPAG8 antibody (Cat. #13915-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice.
Sperm-associated antigens as targets for cancer immunotherapy: expression pattern and humoral immune response in cancer patients.
Merges GE, Meier J, Schneider S, Kruse A, Fröbius AC, Kirfel G, Steger K, Arévalo L, Schorle H
Development (Cambridge, England) 2022 Jun 15;149(12)
Development (Cambridge, England) 2022 Jun 15;149(12)
Sperm-associated antigens as targets for cancer immunotherapy: expression pattern and humoral immune response in cancer patients.
Siliņa K, Zayakin P, Kalniņa Z, Ivanova L, Meistere I, Endzeliņš E, Abols A, Stengrēvics A, Leja M, Ducena K, Kozirovskis V, Linē A
Journal of immunotherapy (Hagerstown, Md. : 1997) 2011 Jan;34(1):28-44
Journal of immunotherapy (Hagerstown, Md. : 1997) 2011 Jan;34(1):28-44
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 13915-1-AP(SPAG8 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-SPAG8 (IP:13915-1-AP, 3ug; Detection:13915-1-AP 1:500) with mouse testis tissue lysate 6000ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human prostate cancer using 13915-1-AP(SPAG8 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SPAG8 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SPAG8. This antibody recognizes human, mouse antigen. The SPAG8 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
