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- Antibody Data
- Antigen structure
- References [23]
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- Product number
- 51012-2-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#51012-2-AP, RRID:AB_2195870
- Product name
- SPTLC2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SPTLC2 antibody (Cat. #51012-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Fresh Ginseng Reduces Sphingosine Secretion Through Ubiquitin Degradation of SPTLC2 to Antagonize the Progression of Non-Small Cell Lung Cancer.
The individual isoforms of ORMDL, the regulatory subunit of serine palmitoyltransferase, have distinctive sensitivities to ceramide.
PLK1-mediated phosphorylation of PHGDH reprograms serine metabolism in advanced prostate cancer.
Extracellular vesicular delivery of ceramides from pulmonary macrophages to endothelial cells facilitates chronic obstructive pulmonary disease.
The individual isoforms of ORMDL, the regulatory subunit of serine palmitoyltransferase, have distinctive sensitivities to ceramide.
Puerarin Targets HIF-1α to Modulate Hypoxia-Related Sphingolipid Metabolism in Diabetic Hepatopathy via the SPTLC2/Ceramide Pathway.
Altered sphingolipid biosynthetic flux and lipoprotein trafficking contribute to trans-fat-induced atherosclerosis.
Glycosphingolipid synthesis mediates immune evasion in KRAS-driven cancer.
ORMDL mislocalization by impaired autophagy in Niemann-Pick type C disease leads to increased de novo sphingolipid biosynthesis.
Ceramide enhanced the hepatic glucagon response through regulation of CREB activity.
m(6)A modification-tuned sphingolipid metabolism regulates postnatal liver development in male mice.
SPTSSA variants alter sphingolipid synthesis and cause a complex hereditary spastic paraplegia.
Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease.
Exercise training enhances muscle mitochondrial metabolism in diet-resistant obesity.
Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice.
IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis.
Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection.
Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function.
Macrophage-specific de Novo Synthesis of Ceramide Is Dispensable for Inflammasome-driven Inflammation and Insulin Resistance in Obesity.
Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.
Magnesium deficiency upregulates serine palmitoyl transferase (SPT 1 and SPT 2) in cardiovascular tissues: relationship to serum ionized Mg and cytochrome c.
Liver-specific deficiency of serine palmitoyltransferase subunit 2 decreases plasma sphingomyelin and increases apolipoprotein E levels.
Serine palmitoyl-CoA transferase (SPT) deficiency and sphingolipid levels in mice.
Jiang R, Wang J, Shou F, Cui J, Huang X, Gao Y, Wan H, Peng Y, Fang Z
Phytotherapy research : PTR 2026 Jan;40(1):227-247
Phytotherapy research : PTR 2026 Jan;40(1):227-247
The individual isoforms of ORMDL, the regulatory subunit of serine palmitoyltransferase, have distinctive sensitivities to ceramide.
Mahawar U, Davis DL, Kannan M, Suemitsu J, Oltorik CD, Farooq F, Fulani R, Weintraub C, Wattenberg B
Biochimica et biophysica acta. Molecular and cell biology of lipids 2025 Oct;1870(7):159677
Biochimica et biophysica acta. Molecular and cell biology of lipids 2025 Oct;1870(7):159677
PLK1-mediated phosphorylation of PHGDH reprograms serine metabolism in advanced prostate cancer.
Rao X, Allison DB, Flight RM, Lin P, He D, Li Z, Zhang Y, Wang R, Li C, Wang J, Wang X, Peng J, Fong KW, Shao Q, Wang C, Ali ES, Moseley HN, Liu X
bioRxiv : the preprint server for biology 2025 May 24;
bioRxiv : the preprint server for biology 2025 May 24;
Extracellular vesicular delivery of ceramides from pulmonary macrophages to endothelial cells facilitates chronic obstructive pulmonary disease.
Huang Q, Kang T, Shen S, Liu L, Zhang L, Zou X, Wu J
Cell communication and signaling : CCS 2025 Mar 7;23(1):124
Cell communication and signaling : CCS 2025 Mar 7;23(1):124
The individual isoforms of ORMDL, the regulatory subunit of serine palmitoyltransferase, have distinctive sensitivities to ceramide.
Mahawar U, Davis DL, Kannan M, Suemitsu J, Oltorik CD, Farooq F, Fulani R, Weintraub C, Allegood J, Wattenberg B
bioRxiv : the preprint server for biology 2025 Mar 21;
bioRxiv : the preprint server for biology 2025 Mar 21;
Puerarin Targets HIF-1α to Modulate Hypoxia-Related Sphingolipid Metabolism in Diabetic Hepatopathy via the SPTLC2/Ceramide Pathway.
Cai M, Lai W, Chen H, Cao D, Zhang B, Wang F, Xian M, Wang S
Pharmaceuticals (Basel, Switzerland) 2025 Mar 12;18(3)
Pharmaceuticals (Basel, Switzerland) 2025 Mar 12;18(3)
Altered sphingolipid biosynthetic flux and lipoprotein trafficking contribute to trans-fat-induced atherosclerosis.
Gengatharan JM, Handzlik MK, Chih ZY, Ruchhoeft ML, Secrest P, Ashley EL, Green CR, Wallace M, Gordts PLSM, Metallo CM
Cell metabolism 2025 Jan 7;37(1):274-290.e9
Cell metabolism 2025 Jan 7;37(1):274-290.e9
Glycosphingolipid synthesis mediates immune evasion in KRAS-driven cancer.
Soula M, Unlu G, Welch R, Chudnovskiy A, Uygur B, Shah V, Alwaseem H, Bunk P, Subramanyam V, Yeh HW, Khan A, Heissel S, Goodarzi H, Victora GD, Beyaz S, Birsoy K
Nature 2024 Sep;633(8029):451-458
Nature 2024 Sep;633(8029):451-458
ORMDL mislocalization by impaired autophagy in Niemann-Pick type C disease leads to increased de novo sphingolipid biosynthesis.
Brown RDR, Mahawar U, Wattenberg BW, Spiegel S
Journal of lipid research 2024 Jun;65(6):100556
Journal of lipid research 2024 Jun;65(6):100556
Ceramide enhanced the hepatic glucagon response through regulation of CREB activity.
Wang J, Wang D, Lu S, Hu Y, Ge Y, Qin X, Mo Y, Kan J, Li D, Zhang R, Liu Y, Zhang WS
Clinical nutrition (Edinburgh, Scotland) 2024 Feb;43(2):366-378
Clinical nutrition (Edinburgh, Scotland) 2024 Feb;43(2):366-378
m(6)A modification-tuned sphingolipid metabolism regulates postnatal liver development in male mice.
Wang S, Chen S, Sun J, Han P, Xu B, Li X, Zhong Y, Xu Z, Zhang P, Mi P, Zhang C, Li L, Zhang H, Xia Y, Li S, Heikenwalder M, Yuan D
Nature metabolism 2023 May;5(5):842-860
Nature metabolism 2023 May;5(5):842-860
SPTSSA variants alter sphingolipid synthesis and cause a complex hereditary spastic paraplegia.
Srivastava S, Shaked HM, Gable K, Gupta SD, Pan X, Somashekarappa N, Han G, Mohassel P, Gotkine M, Doney E, Goldenberg P, Tan QKG, Gong Y, Kleinstiver B, Wishart B, Cope H, Pires CB, Stutzman H, Spillmann RC, Undiagnosed Disease Network, Sadjadi R, Elpeleg O, Lee CH, Bellen HJ, Edvardson S, Eichler F, Dunn TM
Brain : a journal of neurology 2023 Apr 19;146(4):1420-1435
Brain : a journal of neurology 2023 Apr 19;146(4):1420-1435
Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease.
Qi YY, Heng X, Yao ZY, Qu SY, Ge PY, Zhao X, Ni SJ, Guo R, Yang NY, Zhang QC, Zhu HX
Drug design, development and therapy 2022;16:931-950
Drug design, development and therapy 2022;16:931-950
Exercise training enhances muscle mitochondrial metabolism in diet-resistant obesity.
Pileggi CA, Blondin DP, Hooks BG, Parmar G, Alecu I, Patten DA, Cuillerier A, O'Dwyer C, Thrush AB, Fullerton MD, Bennett SA, Doucet É, Haman F, Cuperlovic-Culf M, McPherson R, Dent RRM, Harper ME
EBioMedicine 2022 Sep;83:104192
EBioMedicine 2022 Sep;83:104192
Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice.
Ranea-Robles P, Portman K, Bender A, Lee K, He JC, Mulholland DJ, Argmann C, Houten SM
Kidney360 2021 Sep;2(9):1441-1454
Kidney360 2021 Sep;2(9):1441-1454
IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis.
Dasgupta D, Nakao Y, Mauer AS, Thompson JM, Sehrawat TS, Liao CY, Krishnan A, Lucien F, Guo Q, Liu M, Xue F, Fukushima M, Katsumi T, Bansal A, Pandey MK, Maiers JL, DeGrado T, Ibrahim SH, Revzin A, Pavelko KD, Barry MA, Kaufman RJ, Malhi H
Gastroenterology 2020 Oct;159(4):1487-1503.e17
Gastroenterology 2020 Oct;159(4):1487-1503.e17
Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection.
Orchard RC, Wilen CB, Virgin HW
Nature microbiology 2018 Oct;3(10):1109-1114
Nature microbiology 2018 Oct;3(10):1109-1114
Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function.
Li Z, Kabir I, Tietelman G, Huan C, Fan J, Worgall T, Jiang XC
Cell death & disease 2018 Feb 7;9(2):173
Cell death & disease 2018 Feb 7;9(2):173
Macrophage-specific de Novo Synthesis of Ceramide Is Dispensable for Inflammasome-driven Inflammation and Insulin Resistance in Obesity.
Camell CD, Nguyen KY, Jurczak MJ, Christian BE, Shulman GI, Shadel GS, Dixit VD
The Journal of biological chemistry 2015 Dec 4;290(49):29402-13
The Journal of biological chemistry 2015 Dec 4;290(49):29402-13
Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.
Campbell-Valois FX, Trost M, Chemali M, Dill BD, Laplante A, Duclos S, Sadeghi S, Rondeau C, Morrow IC, Bell C, Gagnon E, Hatsuzawa K, Thibault P, Desjardins M
Molecular & cellular proteomics : MCP 2012 Jul;11(7):M111.016378
Molecular & cellular proteomics : MCP 2012 Jul;11(7):M111.016378
Magnesium deficiency upregulates serine palmitoyl transferase (SPT 1 and SPT 2) in cardiovascular tissues: relationship to serum ionized Mg and cytochrome c.
Altura BM, Shah NC, Li Z, Jiang XC, Perez-Albela JL, Altura BT
American journal of physiology. Heart and circulatory physiology 2010 Sep;299(3):H932-8
American journal of physiology. Heart and circulatory physiology 2010 Sep;299(3):H932-8
Liver-specific deficiency of serine palmitoyltransferase subunit 2 decreases plasma sphingomyelin and increases apolipoprotein E levels.
Li Z, Li Y, Chakraborty M, Fan Y, Bui HH, Peake DA, Kuo MS, Xiao X, Cao G, Jiang XC
The Journal of biological chemistry 2009 Sep 25;284(39):27010-9
The Journal of biological chemistry 2009 Sep 25;284(39):27010-9
Serine palmitoyl-CoA transferase (SPT) deficiency and sphingolipid levels in mice.
Hojjati MR, Li Z, Jiang XC
Biochimica et biophysica acta 2005 Oct 15;1737(1):44-51
Biochimica et biophysica acta 2005 Oct 15;1737(1):44-51
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SPTLC2 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human SPTLC2. This antibody recognizes human, mouse antigen. The SPTLC2 antibody has been validated for the following applications: ELISA, WB analysis.
