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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [4]
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- Product number
- PA5-21142 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPTLC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is NIH-3T3 cell lysate. PA5-21142 can be used with blocking peptide PEP-1256.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Increased de novo ceramide synthesis and accumulation in failing myocardium.
Ji R, Akashi H, Drosatos K, Liao X, Jiang H, Kennel PJ, Brunjes DL, Castillero E, Zhang X, Deng LY, Homma S, George IJ, Takayama H, Naka Y, Goldberg IJ, Schulze PC
JCI insight 2017 May 4;2(9)
JCI insight 2017 May 4;2(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 3T3 cells using a SPT2 polyclonal antibody (Product # PA5-21142) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of 3T3 cells using a SPT2 polyclonal antibody (Product # PA5-21142) at a 10 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of 3T3 cells using SPTLC2 Polyclonal Antibody (Product # PA5-21142) at 10 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 Cells labeling SPT2 with SPTLC2 Polyclonal Antibody (Product # PA5-21142) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
