PA5-117780

antibody from Invitrogen Antibodies

Targeting: ALKBH1 ABH, alkB, ALKBH, hABH

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117780

Provider

Invitrogen Antibodies

Product name

ABH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of ABH1 in Lane A: Jurkat Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117780) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 44 kDa, Observed band size: 44 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ABH1 Polyclonal Antibody (Product # PA5-117780) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ABH1 in human liver with ABH1 Polyclonal Antibody (Product # PA5-117780, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ABH1 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 µL with ABH1 Polyclonal Antibody (Product # PA5-117780) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: ABH1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 43 kDa.