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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MA1-5758 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CENPE Monoclonal Antibody (1H12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This target has a predicted molecular weight of 312 kDa and is only suitable for Western blot if IP is performed first.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1H12
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Deubiquitylase UCHL3 regulates bi-orientation and segregation of chromosomes during mitosis.
Jerabkova K, Liao Y, Kleiss C, Fournane S, Durik M, Agote-Arán A, Brino L, Sedlacek R, Sumara I
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):12751-12767
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):12751-12767
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of CENPE in nocodazole-treated ovarian cancer cells using CENPE monoclonal antibody (Product # MA1-5758). Detection was performed using a fluorescent green-conjugated secondary antibody; DNA was stained using DAPI.
