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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-36529 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aquaporin 5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 36 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references dDAVP Downregulates the AQP3-Mediated Glycerol Transport via V1aR in Human Colon HCT8 Cells.
Differential activation of Ca(2+) influx channels modulate stem cell potency, their proliferation/viability and tissue regeneration.
Diesel Particulate Matter 2.5 Induces Epithelial-to-Mesenchymal Transition and Upregulation of SARS-CoV-2 Receptor during Human Pluripotent Stem Cell-Derived Alveolar Organoid Development.
Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term.
Centrone M, D'Agostino M, Ranieri M, Mola MG, Faviana P, Lippolis PV, Silvestris DA, Venneri M, Di Mise A, Valenti G, Tamma G
Frontiers in cell and developmental biology 2022;10:919438
Frontiers in cell and developmental biology 2022;10:919438
Differential activation of Ca(2+) influx channels modulate stem cell potency, their proliferation/viability and tissue regeneration.
Ahamad N, Sun Y, Nascimento Da Conceicao V, Xavier Paul Ezhilan CRD, Natarajan M, Singh BB
NPJ Regenerative medicine 2021 Oct 20;6(1):67
NPJ Regenerative medicine 2021 Oct 20;6(1):67
Diesel Particulate Matter 2.5 Induces Epithelial-to-Mesenchymal Transition and Upregulation of SARS-CoV-2 Receptor during Human Pluripotent Stem Cell-Derived Alveolar Organoid Development.
Kim JH, Kim J, Kim WJ, Choi YH, Yang SR, Hong SH
International journal of environmental research and public health 2020 Nov 13;17(22)
International journal of environmental research and public health 2020 Nov 13;17(22)
Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term.
Ducza E, Csányi A, Szőke É, Pohóczky K, Hajagos-Tóth J, Kothencz A, Tiszai Z, Gáspár R
Heliyon 2019 Oct;5(10):e02697
Heliyon 2019 Oct;5(10):e02697
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Aquaporin-5 in paraffin-embedded human lung carcinoma using Aquaporin-5 polyclonal antibody (Product # PA5-36529) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of dPM2.5 exposure during hPSC-derived alveolar organoid (AO) development. ( A ) Schematic diagram of AO generation from hPSCs. ( B ) Representative bright field and H&E staining images of AOs. Scale bars, 100 mum. ( C ) Immunofluorescence staining for AEP (EPCAM, CPM, and HOPX, red), AEC1 (AQP5 and T1 alpha , green), AEC2 (SFTPC, red) and mesenchymal stromal cell (VIMENTIN, red) markers in AOs on day 25 of induction. Nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). Scale bars, 100 mum. ( D ) qPCR analysis of the indicated AEP and AEC markers in AOs. Data are shown as fold-change relative to undifferentiated hPSCs (unhPSC). Data is presented as mean +- SD. * p < 0.05, ** p < 0.01. ( E ) Schematic diagram of dPM2.5 treatment during hPSC-derived AO development. Black lines indicate the duration of dPM2.5 treatment. Scale bars, 100 mum. ( F ) Measurement of AO size was taken by averaging the longest and shortest diameter of AOs. ( G , H ) Transcript levels of inflammation, fibrosis, and EMT-related genes were measured using qPCR. Data is presented as mean +- SD. * p < 0.05, ** p < 0.01 (vs. Control).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Expression of channel proteins in salivary glands under various conditions. a Expression of acinar (AQP5, shown as white arrow) and ductal (Na + /K + -ATPase) in control (mock radiated), irradiated, and irradiated + transplanted (2 mM Ca 2+ ) salivary gland tissue sections and b confocal images showing expression of TRPC1 channels in control (mock radiated), irradiated, and irradiated + transplanted salivary gland issue sections. Images were taken using x40 objective and scale bar = 50 mum.
