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antibody from Invitrogen Antibodies
Targeting: CD300A
CMRF-35-H9, CMRF35H, IGSF12, IRC1, IRC2, Irp60
Flow cytometry
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- A15778 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD300a Monoclonal Antibody (MEM-260), PE
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- MEM-260
- Vial size
- 100 Tests
- Storage
- 4° C
Submitted references TGF-β1 potentiates Vγ9Vδ2 T cell adoptive immunotherapy of cancer.
Beatson RE, Parente-Pereira AC, Halim L, Cozzetto D, Hull C, Whilding LM, Martinez O, Taylor CA, Obajdin J, Luu Hoang KN, Draper B, Iqbal A, Hardiman T, Zabinski T, Man F, de Rosales RTM, Xie J, Aswad F, Achkova D, Joseph CR, Ciprut S, Adami A, Roider HG, Hess-Stumpp H, Győrffy B, Quist J, Grigoriadis A, Sommer A, Tutt ANJ, Davies DM, Maher J
Cell reports. Medicine 2021 Dec 21;2(12):100473
Cell reports. Medicine 2021 Dec 21;2(12):100473
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 7 Functional investigation of differentially expressed genes in gammadelta[2] versus gammadelta[T2] cells (A) IL-9 was measured in supernatants harvested on day 15 from gammadelta[2] and gammadelta[T2] cells (values for individual donors are linked by a line; unpaired 2-tailed Student's t test). (B) IL-9 receptor expression was quantified on gammadelta[2] and gammadelta[T2] cells on day 15 using flow cytometry (unpaired 2-tailed Student's t test). (C) PTGER2 protein expression in gammadelta[2] and gammadelta[T2] cells (n = 3 independent donors) was determined by western blotting under reducing conditions. Quantification of band pixel density was performed using ImageJ. Data were normalized for each gammadelta[T2] cell sample with respect to gammadelta[2] cells expanded from the same donor. (D) CD300a was quantified on day 15 from gammadelta[2] and gammadelta[T2] cells using flow cytometry (unpaired Student's t test). (E) The indicated ffLuc-expressing tumor cell lines were co-cultivated with gammadelta[2] and gammadelta[2-ITGAE] cells for 24 h at 1:1 E:T ratio. Tumor cell viability is shown in comparison with tumor cells that had been cultured alone (means +- SDs; 1-way ANOVA). (F) Supernatants were collected from co-cultures described in (E) after 72 h and analyzed for IFN-gamma (means +- SDs; unpaired Student's t test). (G) Effect of exogenous PGE 2 on cytotoxicity (means +- SEMs, n = 10 from 5 independent donors) by gammadelta[2] and gammadelta[T2] cells when co-cul
- Conjugate
- Yellow dye
