PAB29474

antibody from Abnova Corporation

Targeting: TIPIN FLJ20516

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29474

Provider

Abnova Corporation

Product name

TIPIN polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human TIPIN.

Antigen sequence

HEAEDLKMLIRHMEHWAHRLFPKLQFEDFIDRVEY
LGSKKEVQTCLKRIRLDLPILHEDFVSNNDEVAEN
NEHDVTSTELDPFLTNLSESEMFASELS


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with IFITM1 polyclonal antibody (Cat # PAB29474) at 1:500-1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line HEK 293 with IFITM1 polyclonal antibody (Cat # PAB29474) at 1-4 ug/mL concentration shows positivity in nucleus and vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with IFITM1 polyclonal antibody (Cat # PAB29474) shows strong cytoplasmic positivity in lymphoid cells outside reaction centra at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)