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- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [4]
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- Product number
- PA5-26278 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASIC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with chicken, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.33 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Expression Profiles of ASIC1/2 and TRPV1/4 in Common Skin Tumors.
Ackermann K, Wallner S, Brochhausen C, Schreml S
International journal of molecular sciences 2021 Jun 2;22(11)
International journal of molecular sciences 2021 Jun 2;22(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an ACCN2 polyclonal antibody (Product # PA5-26278) in CEM cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CEM cells using an ACCN2 polyclonal antibody (Product # PA5-26278) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Immunohistochemistry of SCC. Immunohistochemical staining for ASIC1, ASIC2, TRPV1 and TRPV4 in SCC tissue. ( a - d ) H&E staining, ( e - h ) immunohistochemical staining, inserted smaller pictures represent a two times larger perspective. Scale bars represent 200 mum. ( a - h ) Patient 8. This SCC shows no expression of ASIC2, only some peritumoral lymphocytes appear positive. The tumor cells show a weak, positive expression of TRPV1 and TRPV4. ASIC1 is expressed strongly on tumor cells. For more stainings of other SCCs, see Supplementary Figures S1-S3 .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Immunohistochemistry of BCC. Immunohistochemical staining for ASIC1, ASIC2, TRPV1 and TRPV4 in BCC tissue. ( a - d ) H&E staining, ( e - h ) immunohistochemical staining, inserted smaller pictures give an overview. Scale bars represent 200 mum. ( a - h ) Patient 10. This BCC shows a strong expression of ASIC1 and TRPV1. The expression of TRPV4 is weak and positive, but this BCC shows no expression of ASIC2. For more stainings of other BCCs, see Supplementary Figures S4-S7 .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Immunohistochemistry of MM. Immunohistochemical staining for ASIC1, ASIC2, TRPV1 and TRPV4 in MM tissue. ( a - d ) Histochemical H&E staining, ( e - h ) immunohistochemical staining, inserted smaller pictures represent a two times larger perspective. Scale bars represent 200 mum. ( a - h ) Patient 30.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Summary of standard immunohistochemical and TMA score results for ASIC1, ASIC2, TRPV1 and TRPV4 on ( a ) SCC, ( b ) BCC, ( c ) NCN and ( d ) MM. ++/green bar: strong positive staining with >80% of cells positive and/or staining intensity is high; +/blue bar: 20-80% of cells show a weak positive/partial positive reaction; -/red bar:
