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- Antibody Data
- Antigen structure
- References [27]
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- Product number
- NB100-64775 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64775, RRID:AB_2220932
- Product name
- Mouse Monoclonal HLA ABC Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-64775 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
- Reactivity
- Human, Mouse, Bovine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.25 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references HLA class I allelic sequence and conformation regulate leukocyte Ig-like receptor binding.
The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways.
Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation.
Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates.
The nature of peptides presented by an HLA class I low expression allele.
Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.
WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.
WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.
The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle.
High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss.
Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes.
Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition.
Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition.
A study of human leukocyte antigen G expression in hydatidiform moles.
Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions.
Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain.
Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast.
A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.
A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.
Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies.
Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies.
Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
Jones DC, Kosmoliaptsis V, Apps R, Lapaque N, Smith I, Kono A, Chang C, Boyle LH, Taylor CJ, Trowsdale J, Allen RL
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 1;186(5):2990-7
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 1;186(5):2990-7
The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways.
Zuo J, Quinn LL, Tamblyn J, Thomas WA, Feederle R, Delecluse HJ, Hislop AD, Rowe M
Journal of virology 2011 Feb;85(4):1604-14
Journal of virology 2011 Feb;85(4):1604-14
Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation.
Vitadello M, Doria A, Tarricone E, Ghirardello A, Gorza L
Arthritis research & therapy 2010;12(2):R52
Arthritis research & therapy 2010;12(2):R52
Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates.
Spentzou A, Bergin P, Gill D, Cheeseman H, Ashraf A, Kaltsidis H, Cashin-Cox M, Anjarwalla I, Steel A, Higgs C, Pozniak A, Piechocka-Trocha A, Wong J, Anzala O, Karita E, Dally L, Gotch F, Walker B, Gilmour J, Hayes P
The Journal of infectious diseases 2010 Mar;201(5):720-9
The Journal of infectious diseases 2010 Mar;201(5):720-9
The nature of peptides presented by an HLA class I low expression allele.
Hinrichs J, Föll D, Bade-Döding C, Huyton T, Blasczyk R, Eiz-Vesper B
Haematologica 2010 Aug;95(8):1373-80
Haematologica 2010 Aug;95(8):1373-80
Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.
Fujita Y, Abe R, Inokuma D, Sasaki M, Hoshina D, Natsuga K, Nishie W, McMillan JR, Nakamura H, Shimizu T, Akiyama M, Sawamura D, Shimizu H
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 10;107(32):14345-50
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 10;107(32):14345-50
WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.
Narita M, Masuko M, Kurasaki T, Kitajima T, Takenouchi S, Saitoh A, Watanabe N, Furukawa T, Toba K, Fuse I, Aizawa Y, Kawakami M, Oka Y, Sugiyama H, Takahashi M
International journal of medical sciences 2010 Apr 20;7(2):72-81
International journal of medical sciences 2010 Apr 20;7(2):72-81
WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.
Narita M, Masuko M, Kurasaki T, Kitajima T, Takenouchi S, Saitoh A, Watanabe N, Furukawa T, Toba K, Fuse I, Aizawa Y, Kawakami M, Oka Y, Sugiyama H, Takahashi M
International journal of medical sciences 2010 Apr 20;7(2):72-81
International journal of medical sciences 2010 Apr 20;7(2):72-81
The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle.
Grotzke JE, Harriff MJ, Siler AC, Nolt D, Delepine J, Lewinsohn DA, Lewinsohn DM
PLoS pathogens 2009 Apr;5(4):e1000374
PLoS pathogens 2009 Apr;5(4):e1000374
High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss.
Koene GJ, Arts-Hilkes YH, van Dijk AJ, van der Ven KJ, Slootweg PJ, de Weger RA, Tilanus MG
Human immunology 2004 Dec;65(12):1455-62
Human immunology 2004 Dec;65(12):1455-62
Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes.
Dressel R, Grzeszik C, Kreiss M, Lindemann D, Herrmann T, Walter L, Günther E
Cancer research 2003 Dec 1;63(23):8212-20
Cancer research 2003 Dec 1;63(23):8212-20
Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition.
Ishitani A, Sageshima N, Lee N, Dorofeeva N, Hatake K, Marquardt H, Geraghty DE
Journal of immunology (Baltimore, Md. : 1950) 2003 Aug 1;171(3):1376-84
Journal of immunology (Baltimore, Md. : 1950) 2003 Aug 1;171(3):1376-84
Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition.
Ishitani A, Sageshima N, Lee N, Dorofeeva N, Hatake K, Marquardt H, Geraghty DE
Journal of immunology (Baltimore, Md. : 1950) 2003 Aug 1;171(3):1376-84
Journal of immunology (Baltimore, Md. : 1950) 2003 Aug 1;171(3):1376-84
A study of human leukocyte antigen G expression in hydatidiform moles.
Goldman-Wohl D, Ariel I, Greenfield C, Hochner-Celnikier D, Lavy Y, Yagel S
American journal of obstetrics and gynecology 2001 Aug;185(2):476-80
American journal of obstetrics and gynecology 2001 Aug;185(2):476-80
Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Yoshino N, Ami Y, Terao K, Tashiro F, Honda M
Experimental animals 2000 Apr;49(2):97-110
Experimental animals 2000 Apr;49(2):97-110
Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Yoshino N, Ami Y, Terao K, Tashiro F, Honda M
Experimental animals 2000 Apr;49(2):97-110
Experimental animals 2000 Apr;49(2):97-110
T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions.
Verbeek MM, Westphal JR, Ruiter DJ, de Waal RM
Journal of immunology (Baltimore, Md. : 1950) 1995 Jun 1;154(11):5876-84
Journal of immunology (Baltimore, Md. : 1950) 1995 Jun 1;154(11):5876-84
Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
Jacobsen CN, Aasted B, Broe MK, Petersen JL
Veterinary immunology and immunopathology 1993 Dec;39(4):461-6
Veterinary immunology and immunopathology 1993 Dec;39(4):461-6
Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
Jacobsen CN, Aasted B, Broe MK, Petersen JL
Veterinary immunology and immunopathology 1993 Dec;39(4):461-6
Veterinary immunology and immunopathology 1993 Dec;39(4):461-6
Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain.
Tanabe M, Sekimata M, Ferrone S, Takiguchi M
Journal of immunology (Baltimore, Md. : 1950) 1992 May 15;148(10):3202-9
Journal of immunology (Baltimore, Md. : 1950) 1992 May 15;148(10):3202-9
Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast.
Stern PL, Beresford N, Friedman CI, Stevens VC, Risk JM, Johnson PM
Journal of immunology (Baltimore, Md. : 1950) 1987 Feb 15;138(4):1088-91
Journal of immunology (Baltimore, Md. : 1950) 1987 Feb 15;138(4):1088-91
A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.
Neefjes JJ, Hensen EJ, de Kroon TI, Ploegh HL
Immunogenetics 1986;23(5):341-7
Immunogenetics 1986;23(5):341-7
A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.
Neefjes JJ, Hensen EJ, de Kroon TI, Ploegh HL
Immunogenetics 1986;23(5):341-7
Immunogenetics 1986;23(5):341-7
Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies.
Brodsky FM, Parham P
Immunogenetics 1982;15(2):151-66
Immunogenetics 1982;15(2):151-66
Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies.
Brodsky FM, Parham P
Immunogenetics 1982;15(2):151-66
Immunogenetics 1982;15(2):151-66
Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A
Cell 1978 May;14(1):9-20
Cell 1978 May;14(1):9-20
Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A
Cell 1978 May;14(1):9-20
Cell 1978 May;14(1):9-20
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Human lymphoblast. Image provided via verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of peripheral blood lymphocytes with anti-HLA-ABC: Low Endotoxin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the PE/Cy5 conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cy5 (NBP1-43073) (blue histogram) or Anti-Human HLA-ABC PE-Cy5 (purple histogram). Cells in the lymphocyte gate were
