11302-1-AP

antibody from Proteintech Group

Targeting: RPL4 L4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

11302-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11302-1-AP, RRID:AB_2181909

Product name

Ribosomal protein L4 antibody

Antibody type

Polyclonal

Description

KD/KO validated Ribosomal protein L4 antibody (Cat. #11302-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Small Nucleolar RNA Snord17 Promotes Self-Renewal of Intestinal Stem Cells through Yy2 mRNA Export and Tead4 Activation.

Zhang P, Xu Y, Lan Y, Xiong Z, Yi Z, Wu R, Li C, Du Y, Guo H, Yang Y, Fan Z
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar;13(13):e12029

---

Pathogenetic mechanisms of muscle-specific ribosomes in dilated cardiomyopathy.

Murphy MR, Ganapathi M, Rotlevi ER, Lee TM, Fisher JM, Patel MV, Jayakar P, Buchanan A, Rippert AL, Ahrens-Nicklas RC, Nair D, Nayak SS, Anand A, Shukla A, Soni RK, Yin Y, Yang F, Garcia EJ, Reilly MP, Chung WK, Wu X
Nature cardiovascular research 2026 Jan;5(1):51-66

---

Profiling of terminating ribosomes reveals translational control at stop codons.

Jia L, Mao Y, Uematsu S, Liu XA, Dong L, de Lima LHF, Qian SB
bioRxiv : the preprint server for biology 2025 Sep 18;

---

TDP-43 dysfunction leads to impaired proteostasis and predisposes mice to worse neurological outcomes after brain injury.

Rotunno MS, Fowler-Magaw M, Zhong J, O'Hara K, Wiggin EA, Cameron D, Stallworth K, Bouley J, McEachern H, Anadolu MN, Nickerson JA, Tapper AR, Molas S, Massi F, Henninger N, King OD, Bosco DA
bioRxiv : the preprint server for biology 2025 Oct 22;

---

Proteomic profiling of primary cilia in the developing brain uncovers new regulators of cortical development.

Liu X, Gutierrez OT, Kaur G, Al-Issa Y, Baboo S, Diedrich JK, Cai E, Yates JR 3rd, Ge X
bioRxiv : the preprint server for biology 2025 May 4;

---

Unveiling the mechanisms of mechanical loading-induced knee osteoarthritis through transcriptomics.

Wu Y, Li A, Sun N, Jiang Z, Li Y, Zhou Z, Li X, Zhao D, Leng X, Dong H
International immunopharmacology 2025 Jun 5;157:114785

---

Inhibition of Heat Shock Protein 90β by Catalpol: A Potential Therapeutic Approach for Alleviating Inflammation-Induced Cartilage Injuries in Osteoarthritis.

Zhou Z, Zhang B, Liu L, Yang J, Wang Y, Lv C, Zhang H, Wei Y, Jiang Z, Peng Z, Zhao D, Leng X, Li X, Su H, Dong H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jul;12(26):e2503909

---

Exploring the Cytoplasmic Retention of CRISPR-Cas9 in Eukaryotic Cells: The Role of Nuclear Localization Signals and Ribosomal Interactions.

Major RM, Mills CA, Xing L, Krantz JL, Wolter JM, Zylka MJ
The CRISPR journal 2025 Apr;8(2):120-136

---

The impact of Coronavirus Nsp1 on host mRNA degradation is independent of its role in translation inhibition.

Bäumlin E, Andenmatten D, Luginbühl J, Lalou A, Schwaller N, Karousis ED
Cell reports 2025 Apr 22;44(4):115488

---

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages.

Susanto TT, Hung V, Levine AG, Chen Y, Kerr CH, Yoo Y, Oses-Prieto JA, Fromm L, Zhang Z, Lantz TC, Fujii K, Wernig M, Burlingame AL, Ruggero D, Barna M
Molecular cell 2024 Sep 19;84(18):3545-3563.e25

---

NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP.

Lv L, Mo J, Qing Y, Wang S, Chen L, Mei A, Xu R, Huang H, Tan J, Li Y, Liu J
Cell reports 2024 Mar 26;43(3):113860

---

Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK.

Li Y, Munoz-Mayorga D, Nie Y, Kang N, Tao Y, Lagerwall J, Pernaci C, Curtin G, Coufal NG, Mertens J, Shi L, Chen X
Cell metabolism 2024 Jun 4;36(6):1351-1370.e8

---

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice.

Zou D, Li K, Su L, Liu J, Lu Y, Huang R, Li M, Mang X, Geng Q, Li P, Tang J, Yu Z, Zhang Z, Chen D, Miao S, Yu J, Yan W, Song W
Developmental cell 2024 Jul 8;59(13):1707-1723.e8

---

CASIN exerts anti-aging effects through RPL4 on the skin of naturally aging mice.

Zhang Y, Wang X, Huang J, Zhang X, Bu L, Zhang Y, Liang F, Wu S, Zhang M, Zhang L, Zhang L
Aging cell 2024 Dec;23(12):e14333

---

Poly-alanine-tailing is a modifier of neurodegeneration caused by Listerin mutation.

Hung HC, Costas-Insua C, Holbrook SE, Stauffer JE, Martin PB, Müller TA, Schroeder DG, Kigoshi-Tansho Y, Xu H, Rudolf R, Cox GA, Joazeiro CAP
bioRxiv : the preprint server for biology 2024 Aug 24;

---

Lysosomal cystine governs ferroptosis sensitivity in cancer via cysteine stress response.

Swanda RV, Ji Q, Wu X, Yan J, Dong L, Mao Y, Uematsu S, Dong Y, Qian SB
Molecular cell 2023 Sep 21;83(18):3347-3359.e9

---

BuShen JianGu Fang alleviates cartilage degeneration via regulating multiple genes and signaling pathways to activate NF-κB/Sox9 axis.

Zhou Z, Lv C, Wang Y, Zhang B, Liu L, Yang J, Leng X, Zhao D, Yao B, Wang J, Dong H
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 May;113:154742

---

RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages.

Susanto TT, Hung V, Levine AG, Kerr CH, Yoo Y, Chen Y, Oses-Prieto JA, Fromm L, Fujii K, Wernig M, Burlingame AL, Ruggero D, Barna M
bioRxiv : the preprint server for biology 2023 Dec 7;

---

m(6)A-modified lincRNA Dubr is required for neuronal development by stabilizing YTHDF1/3 and facilitating mRNA translation.

Huang J, Jiang B, Li GW, Zheng D, Li M, Xie X, Pan Y, Wei M, Liu X, Jiang X, Zhang X, Yang L, Bao L, Wang B
Cell reports 2022 Nov 22;41(8):111693

---

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

Gai X, Xin D, Wu D, Wang X, Chen L, Wang Y, Ma K, Li Q, Li P, Yu X
Cell research 2022 Mar;32(3):254-268

---

The METTL5-TRMT112 N(6)-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.

Sepich-Poore C, Zheng Z, Schmitt E, Wen K, Zhang ZS, Cui XL, Dai Q, Zhu AC, Zhang L, Sanchez Castillo A, Tan H, Peng J, Zhuang X, He C, Nachtergaele S
The Journal of biological chemistry 2022 Mar;298(3):101590

---

Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress.

Shu XE, Mao Y, Jia L, Qian SB
Nature chemical biology 2022 Feb;18(2):134-141

---

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Dabbah M, Lishner M, Jarchowsky-Dolberg O, Tartakover-Matalon S, Brin YS, Pasmanik-Chor M, Neumann A, Drucker L
Translational research : the journal of laboratory and clinical medicine 2021 Oct;236:117-132

---

Hantaviruses use the endogenous host factor P58IPK to combat the PKR antiviral response.

Wang Z, Ren S, Li Q, Royster AD, Lin L, Liu S, Ganaie SS, Qiu J, Mir S, Mir MA
PLoS pathogens 2021 Oct;17(10):e1010007

---

Cilia locally synthesize proteins to sustain their ultrastructure and functions.

Hao K, Chen Y, Yan X, Zhu X
Nature communications 2021 Nov 30;12(1):6971

---

Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation.

Ranjan N, Pochopien AA, Chih-Chien Wu C, Beckert B, Blanchet S, Green R, V Rodnina M, Wilson DN
The EMBO journal 2021 Mar 15;40(6):e106449

---

PRDX2 promotes the proliferation of colorectal cancer cells by increasing the ubiquitinated degradation of p53.

Wang W, Wei J, Zhang H, Zheng X, Zhou H, Luo Y, Yang J, Deng Q, Huang S, Fu Z
Cell death & disease 2021 Jun 11;12(6):605

---

Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.

Schneider K, Nelson GM, Watson JL, Morf J, Dalglish M, Luh LM, Weber A, Bertolotti A
Cell reports 2020 Sep 15;32(11):108154

---

Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay.

Annibaldis G, Domanski M, Dreos R, Contu L, Carl S, Kläy N, Mühlemann O
Nucleic acids research 2020 Oct 9;48(18):10259-10279

---

Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.

Hien A, Molinaro G, Liu B, Huber KM, Richter JD
Molecular autism 2020 Oct 14;11(1):78

---

The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation.

Meyer C, Garzia A, Morozov P, Molina H, Tuschl T
Molecular cell 2020 Mar 19;77(6):1193-1205.e5

---

Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate.

Wu CC, Peterson A, Zinshteyn B, Regot S, Green R
Cell 2020 Jul 23;182(2):404-416.e14

---

Ribosome 18S m(6)A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth.

Rong B, Zhang Q, Wan J, Xing S, Dai R, Li Y, Cai J, Xie J, Song Y, Chen J, Zhang L, Yan G, Zhang W, Gao H, Han JJ, Qu Q, Ma H, Tian Y, Lan F
Cell reports 2020 Dec 22;33(12):108544

---

Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.

Zou M, Zhang X, Jiang D, Zhao Y, Wu T, Gong Q, Su H, Wu D, Moreland L, Li G
Genes 2020 Dec 21;11(12)

---

The Ccr4-Not complex monitors the translating ribosome for codon optimality.

Buschauer R, Matsuo Y, Sugiyama T, Chen YH, Alhusaini N, Sweet T, Ikeuchi K, Cheng J, Matsuki Y, Nobuta R, Gilmozzi A, Berninghausen O, Tesina P, Becker T, Coller J, Inada T, Beckmann R
Science (New York, N.Y.) 2020 Apr 17;368(6488)

---

LncRNA SNHG7 accelerates the proliferation, migration and invasion of hepatocellular carcinoma cells via regulating miR-122-5p and RPL4.

Yang X, Sun L, Wang L, Yao B, Mo H, Yang W
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109386

---

N(6)-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.

Zhou J, Wan J, Shu XE, Mao Y, Liu XM, Yuan X, Zhang X, Hess ME, Brüning JC, Qian SB
Molecular cell 2018 Feb 15;69(4):636-647.e7

---

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Jiang L, Li T, Zhang X, Zhang B, Yu C, Li Y, Fan S, Jiang X, Khan T, Hao Q, Xu P, Nadano D, Huleihel M, Lunenfeld E, Wang PJ, Zhang Y, Shi Q
Current biology : CB 2017 May 22;27(10):1498-1505.e6

---

G3BP-Caprin1-USP10 complexes mediate stress granule condensation and associate with 40S subunits.

Kedersha N, Panas MD, Achorn CA, Lyons S, Tisdale S, Hickman T, Thomas M, Lieberman J, McInerney GM, Ivanov P, Anderson P
The Journal of cell biology 2016 Mar 28;212(7):845-60

---

Characterizing inactive ribosomes in translational profiling.

Liu B, Qian SB
Translation (Austin, Tex.) 2016 Jan-Jun;4(1):e1138018

---

Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18.

Zhang X, Gao X, Coots RA, Conn CS, Liu B, Qian SB
Nature structural & molecular biology 2015 May;22(5):404-10

---

Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice.

Shi Y, Liu X, Li R, Gao Y, Xu Z, Zhang B, Zhou Y
Journal of experimental botany 2014 Jul;65(12):3055-69

---

RNA helicase A is not required for RISC activity.

Liang XH, Crooke ST
Biochimica et biophysica acta 2013 Oct;1829(10):1092-101

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Kinesin molecular motor Eg5 functions during polypeptide synthesis.

Bartoli KM, Jakovljevic J, Woolford JL Jr, Saunders WS
Molecular biology of the cell 2011 Sep;22(18):3420-30

---

Efficient and specific knockdown of small non-coding RNAs in mammalian cells and in mice.

Liang XH, Vickers TA, Guo S, Crooke ST
Nucleic acids research 2011 Feb;39(3):e13

---

Auxin-mediated ribosomal biogenesis regulates vacuolar trafficking in Arabidopsis.

Rosado A, Sohn EJ, Drakakaki G, Pan S, Swidergal A, Xiong Y, Kang BH, Bressan RA, Raikhel NV
The Plant cell 2010 Jan;22(1):143-58

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11302-1-AP(RPL4 antibody) at dilution of 1:300

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using RPL4 antibody 11302-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-RPL4 (IP:11302-1-AP, 3ug; Detection:11302-1-AP 1:800) with K-562 cells lysate 6000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lymphoma using 11302-1-AP(RPL4 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue