Antibody data
- Antibody Data
- Antigen structure
- References [33]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 11302-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11302-1-AP, RRID:AB_2181909
- Product name
- Ribosomal protein L4 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Ribosomal protein L4 antibody (Cat. #11302-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP.
Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK.
Lysosomal cystine governs ferroptosis sensitivity in cancer via cysteine stress response.
BuShen JianGu Fang alleviates cartilage degeneration via regulating multiple genes and signaling pathways to activate NF-κB/Sox9 axis.
RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages.
m(6)A-modified lincRNA Dubr is required for neuronal development by stabilizing YTHDF1/3 and facilitating mRNA translation.
Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.
The METTL5-TRMT112 N(6)-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.
Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress.
Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.
Hantaviruses use the endogenous host factor P58IPK to combat the PKR antiviral response.
Cilia locally synthesize proteins to sustain their ultrastructure and functions.
Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation.
PRDX2 promotes the proliferation of colorectal cancer cells by increasing the ubiquitinated degradation of p53.
Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.
Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay.
Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.
The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation.
Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate.
Ribosome 18S m(6)A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth.
Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.
The Ccr4-Not complex monitors the translating ribosome for codon optimality.
LncRNA SNHG7 accelerates the proliferation, migration and invasion of hepatocellular carcinoma cells via regulating miR-122-5p and RPL4.
N(6)-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.
RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.
G3BP-Caprin1-USP10 complexes mediate stress granule condensation and associate with 40S subunits.
Characterizing inactive ribosomes in translational profiling.
Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18.
Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice.
RNA helicase A is not required for RISC activity.
Kinesin molecular motor Eg5 functions during polypeptide synthesis.
Efficient and specific knockdown of small non-coding RNAs in mammalian cells and in mice.
Auxin-mediated ribosomal biogenesis regulates vacuolar trafficking in Arabidopsis.
Lv L, Mo J, Qing Y, Wang S, Chen L, Mei A, Xu R, Huang H, Tan J, Li Y, Liu J
Cell reports 2024 Mar 26;43(3):113860
Cell reports 2024 Mar 26;43(3):113860
Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK.
Li Y, Munoz-Mayorga D, Nie Y, Kang N, Tao Y, Lagerwall J, Pernaci C, Curtin G, Coufal NG, Mertens J, Shi L, Chen X
Cell metabolism 2024 Jun 4;36(6):1351-1370.e8
Cell metabolism 2024 Jun 4;36(6):1351-1370.e8
Lysosomal cystine governs ferroptosis sensitivity in cancer via cysteine stress response.
Swanda RV, Ji Q, Wu X, Yan J, Dong L, Mao Y, Uematsu S, Dong Y, Qian SB
Molecular cell 2023 Sep 21;83(18):3347-3359.e9
Molecular cell 2023 Sep 21;83(18):3347-3359.e9
BuShen JianGu Fang alleviates cartilage degeneration via regulating multiple genes and signaling pathways to activate NF-κB/Sox9 axis.
Zhou Z, Lv C, Wang Y, Zhang B, Liu L, Yang J, Leng X, Zhao D, Yao B, Wang J, Dong H
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 May;113:154742
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 May;113:154742
RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages.
Susanto TT, Hung V, Levine AG, Kerr CH, Yoo Y, Chen Y, Oses-Prieto JA, Fromm L, Fujii K, Wernig M, Burlingame AL, Ruggero D, Barna M
bioRxiv : the preprint server for biology 2023 Dec 7;
bioRxiv : the preprint server for biology 2023 Dec 7;
m(6)A-modified lincRNA Dubr is required for neuronal development by stabilizing YTHDF1/3 and facilitating mRNA translation.
Huang J, Jiang B, Li GW, Zheng D, Li M, Xie X, Pan Y, Wei M, Liu X, Jiang X, Zhang X, Yang L, Bao L, Wang B
Cell reports 2022 Nov 22;41(8):111693
Cell reports 2022 Nov 22;41(8):111693
Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.
Gai X, Xin D, Wu D, Wang X, Chen L, Wang Y, Ma K, Li Q, Li P, Yu X
Cell research 2022 Mar;32(3):254-268
Cell research 2022 Mar;32(3):254-268
The METTL5-TRMT112 N(6)-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.
Sepich-Poore C, Zheng Z, Schmitt E, Wen K, Zhang ZS, Cui XL, Dai Q, Zhu AC, Zhang L, Sanchez Castillo A, Tan H, Peng J, Zhuang X, He C, Nachtergaele S
The Journal of biological chemistry 2022 Mar;298(3):101590
The Journal of biological chemistry 2022 Mar;298(3):101590
Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress.
Shu XE, Mao Y, Jia L, Qian SB
Nature chemical biology 2022 Feb;18(2):134-141
Nature chemical biology 2022 Feb;18(2):134-141
Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.
Dabbah M, Lishner M, Jarchowsky-Dolberg O, Tartakover-Matalon S, Brin YS, Pasmanik-Chor M, Neumann A, Drucker L
Translational research : the journal of laboratory and clinical medicine 2021 Oct;236:117-132
Translational research : the journal of laboratory and clinical medicine 2021 Oct;236:117-132
Hantaviruses use the endogenous host factor P58IPK to combat the PKR antiviral response.
Wang Z, Ren S, Li Q, Royster AD, Lin L, Liu S, Ganaie SS, Qiu J, Mir S, Mir MA
PLoS pathogens 2021 Oct;17(10):e1010007
PLoS pathogens 2021 Oct;17(10):e1010007
Cilia locally synthesize proteins to sustain their ultrastructure and functions.
Hao K, Chen Y, Yan X, Zhu X
Nature communications 2021 Nov 30;12(1):6971
Nature communications 2021 Nov 30;12(1):6971
Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation.
Ranjan N, Pochopien AA, Chih-Chien Wu C, Beckert B, Blanchet S, Green R, V Rodnina M, Wilson DN
The EMBO journal 2021 Mar 15;40(6):e106449
The EMBO journal 2021 Mar 15;40(6):e106449
PRDX2 promotes the proliferation of colorectal cancer cells by increasing the ubiquitinated degradation of p53.
Wang W, Wei J, Zhang H, Zheng X, Zhou H, Luo Y, Yang J, Deng Q, Huang S, Fu Z
Cell death & disease 2021 Jun 11;12(6):605
Cell death & disease 2021 Jun 11;12(6):605
Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation.
Schneider K, Nelson GM, Watson JL, Morf J, Dalglish M, Luh LM, Weber A, Bertolotti A
Cell reports 2020 Sep 15;32(11):108154
Cell reports 2020 Sep 15;32(11):108154
Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay.
Annibaldis G, Domanski M, Dreos R, Contu L, Carl S, Kläy N, Mühlemann O
Nucleic acids research 2020 Oct 9;48(18):10259-10279
Nucleic acids research 2020 Oct 9;48(18):10259-10279
Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.
Hien A, Molinaro G, Liu B, Huber KM, Richter JD
Molecular autism 2020 Oct 14;11(1):78
Molecular autism 2020 Oct 14;11(1):78
The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation.
Meyer C, Garzia A, Morozov P, Molina H, Tuschl T
Molecular cell 2020 Mar 19;77(6):1193-1205.e5
Molecular cell 2020 Mar 19;77(6):1193-1205.e5
Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate.
Wu CC, Peterson A, Zinshteyn B, Regot S, Green R
Cell 2020 Jul 23;182(2):404-416.e14
Cell 2020 Jul 23;182(2):404-416.e14
Ribosome 18S m(6)A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth.
Rong B, Zhang Q, Wan J, Xing S, Dai R, Li Y, Cai J, Xie J, Song Y, Chen J, Zhang L, Yan G, Zhang W, Gao H, Han JJ, Qu Q, Ma H, Tian Y, Lan F
Cell reports 2020 Dec 22;33(12):108544
Cell reports 2020 Dec 22;33(12):108544
Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.
Zou M, Zhang X, Jiang D, Zhao Y, Wu T, Gong Q, Su H, Wu D, Moreland L, Li G
Genes 2020 Dec 21;11(12)
Genes 2020 Dec 21;11(12)
The Ccr4-Not complex monitors the translating ribosome for codon optimality.
Buschauer R, Matsuo Y, Sugiyama T, Chen YH, Alhusaini N, Sweet T, Ikeuchi K, Cheng J, Matsuki Y, Nobuta R, Gilmozzi A, Berninghausen O, Tesina P, Becker T, Coller J, Inada T, Beckmann R
Science (New York, N.Y.) 2020 Apr 17;368(6488)
Science (New York, N.Y.) 2020 Apr 17;368(6488)
LncRNA SNHG7 accelerates the proliferation, migration and invasion of hepatocellular carcinoma cells via regulating miR-122-5p and RPL4.
Yang X, Sun L, Wang L, Yao B, Mo H, Yang W
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109386
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109386
N(6)-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.
Zhou J, Wan J, Shu XE, Mao Y, Liu XM, Yuan X, Zhang X, Hess ME, Brüning JC, Qian SB
Molecular cell 2018 Feb 15;69(4):636-647.e7
Molecular cell 2018 Feb 15;69(4):636-647.e7
RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.
Jiang L, Li T, Zhang X, Zhang B, Yu C, Li Y, Fan S, Jiang X, Khan T, Hao Q, Xu P, Nadano D, Huleihel M, Lunenfeld E, Wang PJ, Zhang Y, Shi Q
Current biology : CB 2017 May 22;27(10):1498-1505.e6
Current biology : CB 2017 May 22;27(10):1498-1505.e6
G3BP-Caprin1-USP10 complexes mediate stress granule condensation and associate with 40S subunits.
Kedersha N, Panas MD, Achorn CA, Lyons S, Tisdale S, Hickman T, Thomas M, Lieberman J, McInerney GM, Ivanov P, Anderson P
The Journal of cell biology 2016 Mar 28;212(7):845-60
The Journal of cell biology 2016 Mar 28;212(7):845-60
Characterizing inactive ribosomes in translational profiling.
Liu B, Qian SB
Translation (Austin, Tex.) 2016 Jan-Jun;4(1):e1138018
Translation (Austin, Tex.) 2016 Jan-Jun;4(1):e1138018
Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18.
Zhang X, Gao X, Coots RA, Conn CS, Liu B, Qian SB
Nature structural & molecular biology 2015 May;22(5):404-10
Nature structural & molecular biology 2015 May;22(5):404-10
Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice.
Shi Y, Liu X, Li R, Gao Y, Xu Z, Zhang B, Zhou Y
Journal of experimental botany 2014 Jul;65(12):3055-69
Journal of experimental botany 2014 Jul;65(12):3055-69
RNA helicase A is not required for RISC activity.
Liang XH, Crooke ST
Biochimica et biophysica acta 2013 Oct;1829(10):1092-101
Biochimica et biophysica acta 2013 Oct;1829(10):1092-101
Kinesin molecular motor Eg5 functions during polypeptide synthesis.
Bartoli KM, Jakovljevic J, Woolford JL Jr, Saunders WS
Molecular biology of the cell 2011 Sep;22(18):3420-30
Molecular biology of the cell 2011 Sep;22(18):3420-30
Efficient and specific knockdown of small non-coding RNAs in mammalian cells and in mice.
Liang XH, Vickers TA, Guo S, Crooke ST
Nucleic acids research 2011 Feb;39(3):e13
Nucleic acids research 2011 Feb;39(3):e13
Auxin-mediated ribosomal biogenesis regulates vacuolar trafficking in Arabidopsis.
Rosado A, Sohn EJ, Drakakaki G, Pan S, Swidergal A, Xiong Y, Kang BH, Bressan RA, Raikhel NV
The Plant cell 2010 Jan;22(1):143-58
The Plant cell 2010 Jan;22(1):143-58
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11302-1-AP(RPL4 antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RPL4 antibody 11302-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-RPL4 (IP:11302-1-AP, 3ug; Detection:11302-1-AP 1:800) with K-562 cells lysate 6000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lymphoma using 11302-1-AP(RPL4 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue