NBP2-47126

antibody from Novus Biologicals

Targeting: GLIPR2 C9orf19, GAPR-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-47126

Provider

Novus Biologicals

Product name

Rabbit Polyclonal GLIPR2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GLIPR2 Antibody [NBP2-47126] - Total protein from human liver (lane 1) and human spleen (lane 2) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-GAPR1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and hematoxylin counterstaining. GLIPR2 is a protein which localizes to Golgi membrane as well as extracellular exosomes, and this GLIPR2 antibody generated a very specific cytoplasmic staining with strong signal in extracellular spaces/around cellular membranes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GLIPR2 Antibody [NBP2-47126] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using GLIPR2 antibody at 1:200 dilution with HRP-DAB detection and hematoxylin counterstaining. GLIPR2 is a protein which localizes to Golgi membrane as well as extracellular exosomes, and this GLIPR2 antibody generated a very specific cytoplasmic staining with strong signal in extracellular spaces/around cellular membranes.