15450-1-AP

antibody from Proteintech Group

Targeting: GET3 ARSA-I, ASNA1, TRC40

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

15450-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15450-1-AP, RRID:AB_2060110

Product name

ASNA1 antibody

Antibody type

Polyclonal

Description

KD/KO validated ASNA1 antibody (Cat. #15450-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.

Hirata T, Yang J, Tomida S, Tokoro Y, Kinoshita T, Fujita M, Kizuka Y
The Journal of biological chemistry 2022 Oct;298(10):102444

---

Human SND2 mediates ER targeting of GPI-anchored proteins with low hydrophobic GPI attachment signals.

Yang J, Hirata T, Liu YS, Guo XY, Gao XD, Kinoshita T, Fujita M
FEBS letters 2021 Jun;595(11):1542-1558

---

An alternative membrane topology permits lipid droplet localization of peroxisomal fatty acyl-CoA reductase 1.

Exner T, Romero-Brey I, Yifrach E, Rivera-Monroy J, Schrul B, Zouboulis CC, Stremmel W, Honsho M, Bartenschlager R, Zalckvar E, Poppelreuther M, Füllekrug J
Journal of cell science 2019 Mar 18;132(6)

---

A trap mutant reveals the physiological client spectrum of TRC40.

Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science 2019 Jul 1;132(13)

---

Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Pfaff J, Rivera Monroy J, Jamieson C, Rajanala K, Vilardi F, Schwappach B, Kehlenbach RH
Journal of cell science 2016 Feb 1;129(3):502-16

---

Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.

Yamamoto Y, Sakisaka T
Molecular cell 2012 Nov 9;48(3):387-97

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP(ASNA1 antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ASNA1 antibody 15450-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 15450-1-AP(ASNA1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 15450-1-AP(ASNA1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue