Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-22323 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal GOLGB1/Giantin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GOLGB1/Giantin Antibody [NBP2-22323] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22323 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22323] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22323 used for IP at 6 ug/mg lysate. Giantin was also immunoprecipitated by rabbit anti-Giantin antibodies NBP2-22321 and NBP2-22322. For blotting immunoprecipitated Giantin, NBP2-22323 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.