AF8159

antibody from R&D Systems

Targeting: GOLGB1 GCP, GCP372, giantin, GOLIM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF8159

Provider

R&D Systems

Product name

Human/Rat GOLGB1/Giantin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human GOLGB1/Giantin in direct ELISAs. Detects human and rat GOLGB1/Giantin in Western blots.

Reactivity

Human, Rat

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q14789


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Rat GOLGB1/Giantin by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, SK-OV-3 human ovarian adenocarcinoma cell line, ZR-75 human breast cancer cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GOLGB1/Giantin at approximately 370 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

GOLGB1/Giantin in HeLa Human Cell Line. GOLGB1/Giantin was detected in 2% paraformaldehyde immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.