PA5-42884

antibody from Invitrogen Antibodies

Targeting: GOLGB1 GCP, GCP372, giantin, GOLIM1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-42884

Provider

Invitrogen Antibodies

Product name

GOLGB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: NKYIEEMKAQ GGTVLPTEPQ SEEQLSKHDK SSTEEEMEIE KIKHKLQEKE Sequence homology: Cow: 100%; Dog: 100%; Horse: 93%; Human: 100%; Mouse: 85%; Pig: 100%; Rabbit: 100%; Rat: 92%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Towards Age-Related Anti-Inflammatory Therapy: Klotho Suppresses Activation of ER and Golgi Stress Response in Senescent Monocytes.

Mytych J, Sołek P, Będzińska A, Rusinek K, Warzybok A, Tabęcka-Łonczyńska A, Koziorowski M
Cells 2020 Jan 21;9(2)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of human uterus tissue using an anti-GOLGB1 polyclonal antibody (Product # PA5-42884).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Surface Plasmon Resonance of GOLGB1 polyclonal antibody (Product # PA5-42884). Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 µg/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333 nm peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.