Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ALX-804-600-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-600-C100, RRID:AB_2051486
- Product name
- Giantin monoclonal antibody (G1/133)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Affinity purified (HYDRA column) and gel filtrated (FPLC).
- Reactivity
- Chicken/Avian, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- G1/133
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Substrate requirements for SPPL2b-dependent regulated intramembrane proteolysis.
The cargo receptors Surf4, endoplasmic reticulum-Golgi intermediate compartment (ERGIC)-53, and p25 are required to maintain the architecture of ERGIC and Golgi.
Capturing protein interactions in the secretory pathway of living cells.
Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46.
Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme.
Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway.
Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa.
Martin L, Fluhrer R, Haass C
The Journal of biological chemistry 2009 Feb 27;284(9):5662-70
The Journal of biological chemistry 2009 Feb 27;284(9):5662-70
The cargo receptors Surf4, endoplasmic reticulum-Golgi intermediate compartment (ERGIC)-53, and p25 are required to maintain the architecture of ERGIC and Golgi.
Mitrovic S, Ben-Tekaya H, Koegler E, Gruenberg J, Hauri HP
Molecular biology of the cell 2008 May;19(5):1976-90
Molecular biology of the cell 2008 May;19(5):1976-90
Capturing protein interactions in the secretory pathway of living cells.
Nyfeler B, Michnick SW, Hauri HP
Proceedings of the National Academy of Sciences of the United States of America 2005 May 3;102(18):6350-5
Proceedings of the National Academy of Sciences of the United States of America 2005 May 3;102(18):6350-5
Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46.
Breuza L, Halbeisen R, Jenö P, Otte S, Barlowe C, Hong W, Hauri HP
The Journal of biological chemistry 2004 Nov 5;279(45):47242-53
The Journal of biological chemistry 2004 Nov 5;279(45):47242-53
Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme.
Vollenweider F, Kappeler F, Itin C, Hauri HP
The Journal of cell biology 1998 Jul 27;142(2):377-89
The Journal of cell biology 1998 Jul 27;142(2):377-89
Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway.
Itin C, Schindler R, Hauri HP
The Journal of cell biology 1995 Oct;131(1):57-67
The Journal of cell biology 1995 Oct;131(1):57-67
Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa.
Linstedt AD, Hauri HP
Molecular biology of the cell 1993 Jul;4(7):679-93
Molecular biology of the cell 1993 Jul;4(7):679-93
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunocytochemistry using MAb to Giantin (G1/133) (Prod. No. ALX-804-600) at a 1:1'000 dilution.
