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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-31051 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549. Predicted reactivity: Mouse (91%), Rat (91%), Pig (91%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.51 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Hepatic AQP9 expression in male rats is reduced in response to PPARĪ± agonist treatment.
Lebeck J, Cheema MU, Skowronski MT, Nielsen S, Praetorius J
American journal of physiology. Gastrointestinal and liver physiology 2015 Feb 1;308(3):G198-205
American journal of physiology. Gastrointestinal and liver physiology 2015 Feb 1;308(3):G198-205
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GPD1 Polyclonal Antibody (Product # PA5-31051). Sample (30 µg of whole cell lysate). Lane A: A549. 10% SDS PAGE. GPD1 Polyclonal Antibody (Product # PA5-31051) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GPD1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GPD1 Polyclonal Antibody (Product # PA5-31051) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPD1 Polyclonal Antibody detects GPD1 protein at cytosol on D54MG xenograft by immunohistochemical analysis. Sample: Paraffin-embedded D54MG xenograft. GPD1 Polyclonal Antibody (Product # PA5-31051) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPD1 Polyclonal Antibody detects GPD1 protein at cytosol on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. GPD1 Polyclonal Antibody (Product # PA5-31051) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GPD1 (Product # PA5-31051) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
