LS-C742438

antibody from LSBio

Targeting: SDF4 Cab45

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C742438

Provider

LSBio

Product name

SDF4 Antibody (aa161-175, Biotin) LS-C742438

Antibody type

Polyclonal

Description

ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Reactivity

Human

Host

Goat

Conjugate

Biotin

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

SDF4 biotinylated antibody (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

SDF4 biotinylated antibody (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

SDF4 biotinylated antibody (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.