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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- PA5-90470 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SDF4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RPANHSSTRE RVANREENEI LPPDHLNGVK LEMDGHLNRG FHQEVFLGKD LGGFDEDAEP RRSRRKLMVI FSKVDVNTDR KISAKEMQRW IMEKTAEHFQ EAMEESKTHF RAVDPDGDGH VSWDEYKVKF LASKGHSEKE VADAIRLNEE LKVDEETQEV LENL; Positive Samples: DU145, Mouse brain; Cellular Location: Cell projection, Cytoplasm, Golgi apparatus lumen, bleb
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.92 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SDF4 in extracts of various cell lines. Samples were incubated with SDF4 Polyclonal antibody (Product # PA5-90470) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SDF4 in paraffin-embedded human colon. Samples were incubated with SDF4 Polyclonal antibody (Product # PA5-90470) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SDF4 in paraffin-embedded human liver. Samples were incubated with SDF4 Polyclonal antibody (Product # PA5-90470) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SDF4 in paraffin-embedded human liver. Samples were incubated with SDF4 Polyclonal antibody (Product # PA5-90470) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
