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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-117367 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR2K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Centrifuge briefly prior to opening.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1.58 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of POLR2K in SH-SY5Y whole cell and nuclear extracts (30 µg). Samples were separated with 15% SDS-PAGE and incubated in POLR2K polyclonal antibody (Product # PA5-117367) using a dilution of 1:1,000, followed by HRP-conjugated anti-rabbit IgG. Detection was performed with Trident ECL plus-Enhanced.
