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Western blot
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- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00009804-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009804-M01, RRID:AB_2303717
- Product name
- Mouse Monoclonal TOMM20 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TOMM20 - translocase of outer mitochondrial membrane 20 homolog (yeast)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Mitophagy in Refractory Temporal Lobe Epilepsy Patients with Hippocampal Sclerosis.
Neuronal Preconditioning Requires the Mitophagic Activity of C-terminus of HSC70-Interacting Protein.
TBC1D20 mediates autophagy as a key regulator of autophagosome maturation.
Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease.
Mitotic redistribution of the mitochondrial network by Miro and Cenp-F.
Short RNA molecules with high binding affinity to the KH motif of A-kinase anchoring protein 1 (AKAP1): implications for the regulation of steroidogenesis.
Cellular distribution and subcellular localization of spatacsin and spastizin, two proteins involved in hereditary spastic paraplegia.
Wu M, Liu X, Chi X, Zhang L, Xiong W, Chiang SMV, Zhou D, Li J
Cellular and molecular neurobiology 2018 Mar;38(2):479-486
Cellular and molecular neurobiology 2018 Mar;38(2):479-486
Neuronal Preconditioning Requires the Mitophagic Activity of C-terminus of HSC70-Interacting Protein.
Lizama BN, Palubinsky AM, Raveendran VA, Moore AM, Federspiel JD, Codreanu SG, Liebler DC, McLaughlin B
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Aug 1;38(31):6825-6840
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Aug 1;38(31):6825-6840
TBC1D20 mediates autophagy as a key regulator of autophagosome maturation.
Sidjanin DJ, Park AK, Ronchetti A, Martins J, Jackson WT
Autophagy 2016 Oct 2;12(10):1759-1775
Autophagy 2016 Oct 2;12(10):1759-1775
Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease.
Lood C, Blanco LP, Purmalek MM, Carmona-Rivera C, De Ravin SS, Smith CK, Malech HL, Ledbetter JA, Elkon KB, Kaplan MJ
Nature medicine 2016 Feb;22(2):146-53
Nature medicine 2016 Feb;22(2):146-53
Mitotic redistribution of the mitochondrial network by Miro and Cenp-F.
Kanfer G, Courthéoux T, Peterka M, Meier S, Soste M, Melnik A, Reis K, Aspenström P, Peter M, Picotti P, Kornmann B
Nature communications 2015 Aug 11;6:8015
Nature communications 2015 Aug 11;6:8015
Short RNA molecules with high binding affinity to the KH motif of A-kinase anchoring protein 1 (AKAP1): implications for the regulation of steroidogenesis.
Grozdanov PN, Stocco DM
Molecular endocrinology (Baltimore, Md.) 2012 Dec;26(12):2104-17
Molecular endocrinology (Baltimore, Md.) 2012 Dec;26(12):2104-17
Cellular distribution and subcellular localization of spatacsin and spastizin, two proteins involved in hereditary spastic paraplegia.
Murmu RP, Martin E, Rastetter A, Esteves T, Muriel MP, El Hachimi KH, Denora PS, Dauphin A, Fernandez JC, Duyckaerts C, Brice A, Darios F, Stevanin G
Molecular and cellular neurosciences 2011 Jul;47(3):191-202
Molecular and cellular neurosciences 2011 Jul;47(3):191-202
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of TOMM20 expression in transfected 293T cell line by TOMM20 monoclonal antibody (M01), clone 4F3.Lane 1: TOMM20 transfected lysate(16.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - TOMM20 monoclonal antibody (M01), clone 4F3 Analysis of TOMM20 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TOMM20 Antibody (4F3) [H00009804-M01] - Detection limit for recombinant GST tagged TOMM20 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of monoclonal antibody to TOMM20 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of monoclonal antibody to TOMM20 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
