Explore
Validate
Learn
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [5]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-34964 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOM20 Recombinant Rabbit Monoclonal Antibody (10G6I8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YCIYFDRKRR SDPNFKNRLR ERRKKQKLAK ERAGLSKLPD LKDAEAVQKF FLEEIQLGEE LLAQGEYEKG VDHLTNAIAV CGQPQQLLQV LQQTLPPPVF QMLLTKLPTI SQRIVSAQSL AEDDVE
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 10G6I8
- Vial size
- 100 µL
- Concentration
- 1.10 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references miR-210 Regulates Apoptotic Cell Death during Cellular Hypoxia and Reoxygenation in a Diametrically Opposite Manner.
Marwarha G, Røsand Ø, Scrimgeour N, Slagsvold KH, Høydal MA
Biomedicines 2021 Dec 25;10(1)
Biomedicines 2021 Dec 25;10(1)
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TOM20 in C6 cells. Samples were incubated in monoclonal TOM20 antibody (Product # MA5-34964) using a dilution of 1:100, followed by DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TOM20 in HeLa cells. Samples were incubated in monoclonal TOM20 antibody (Product # MA5-34964) using a dilution of 1:100, followed by DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TOM20 in NIH/3T3 cells. Samples were incubated in monoclonal TOM20 antibody (Product # MA5-34964) using a dilution of 1:100, followed by DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TOM20 in NIH/3T3 cells. Samples were incubated in monoclonal TOM20 antibody (Product # MA5-34964) using a dilution of 1:100, followed by DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal imaging (Immunocytochemistry) of TOM20 in HeLa cells. Samples were incubated with TOM20 Monoclonal antibody (Product # MA5-34964) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TOM20 in 200 μg extracts from HeLa cells. Samples were precipitated with 3 μg TOM20 Monoclonal antibody (Product # MA5-34964). Western blot was performed from the immunoprecipitate using TOM20 Monoclonal antibody (Product # MA5-34964) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TOM20 in paraffin-embedded rat heart. Samples were incubated with TOM20 Monoclonal antibody (Product # MA5-34964) using a dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TOM20 in paraffin-embedded human colon carcinoma. Samples were incubated with TOM20 Monoclonal antibody (Product # MA5-34964) using a dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TOM20 in paraffin-embedded human placenta. Samples were incubated with TOM20 Monoclonal antibody (Product # MA5-34964) using a dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TOM20 in paraffin-embedded mouse kidney. Samples were incubated with TOM20 Monoclonal antibody (Product # MA5-34964) using a dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of TOM20 in HeLa cells. Samples were incubated in monoclonal TOM20 antibody (Product # MA5-34964) using a dilution of 1:1000.
