HPA011562

antibody from Atlas Antibodies

Targeting: TOMM20 KIAA0016, MAS20, MOM19, TOM20

Provider product page for HPA011562

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [49]
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Validations
Immunocytochemistry [1]

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Product number: HPA011562 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA011562, RRID:AB_1080326
Product name: Anti-TOMM20
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human TOMM20, Gene description: translocase of outer mitochondrial membrane 20 homolog (yeast), Alternative Gene Names: KIAA0016, MAS20, MOM19, TOM20, Validated applications: ICC, IHC, WB, Uniprot ID: Q15388, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.1 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

TOMM20 protein structure - HPA011562 shown in red.

Submitted references The P2X7 Receptor is a Master Regulator of Microparticle and Mitochondria Exchange in Mouse Microglia

Falzoni S, Vultaggio-Poma V, Chiozzi P, Tarantini M, Adinolfi E, Boldrini P, Giuliani A, Morciano G, Tang Y, Gorecki D, Di Virgilio F
Function 2024;5(4)

Acetalax (Oxyphenisatin Acetate, NSC 59687) and Bisacodyl Cause Oncosis in Triple-Negative Breast Cancer Cell Lines by Poisoning the Ion Exchange Membrane Protein TRPM4

Mizunuma M, Redon C, Saha L, Tran A, Dhall A, Sebastian R, Taniyama D, Kruhlak M, Reinhold W, Takebe N, Pommier Y
Cancer Research Communications 2024;4(8):2101-2111

de Calbiac H, Montealegre S, Straube M, Renault S, Debruge H, Chentout L, Ciura S, Imbard A, Le Guillou E, Marian A, Goudin N, Caccavelli L, Fabrega S, Hubas A, van Endert P, Dupont N, Diana J, Kabashi E, de Lonlay P
Autophagy Reports 2024;3(1)

MAPK1 Mediates MAM Disruption and Mitochondrial Dysfunction in Diabetic Kidney Disease via the PACS-2-Dependent Mechanism

Liu S, Han S, Wang C, Chen H, Xu Q, Feng S, Wang Y, Yao J, Zhou Q, Tang X, Lin L, Hu L, Davidson A, Yang B, Ye C, Yang F, Mao J, Tong C, Chen J, Jiang H
International Journal of Biological Sciences 2024;20(2):569-584

Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects

de Calbiac H, Renault S, Haouy G, Jung V, Roger K, Zhou Q, Campanari M, Chentout L, Demy D, Marian A, Goudin N, Edbauer D, Guerrera C, Ciura S, Kabashi E
Autophagy 2024;20(10):2164-2185

Quantifying nanoscopic alterations associated with mitochondrial dysfunction using three-dimensional single-molecule localization microscopy

Brenner B, Xu F, Zhang Y, Kweon J, Fang R, Sheibani N, Zhang S, Sun C, Zhang H
Biomedical Optics Express 2024;15(3):1571

Neuronal extracellular vesicles influence the expression, degradation and oligomeric state of fructose 1,6-bisphosphatase 2 in astrocytes affecting their glycolytic capacity

Hajka D, Budziak B, Rakus D, Gizak A
Scientific Reports 2024;14(1)

Yeo W, Brenner B, Lee Y, Kweon J, Sun C, Zhang H
2024

Segregation of pathways leading to pexophagy

Barone F, Urbé S, Clague M
Life Science Alliance 2023;6(5):e202201825

Mitochondrial rewiring drives metabolic adaptation to NAD(H) shortage in triple negative breast cancer cells

Carreira A, Ravera S, Zucal C, Thongon N, Irene C, Astigiano C, Bertola N, Buongiorno A, Roccuzzo M, Bisio A, Pardini B, Nencioni A, Bruzzone S, Provenzani A
Neoplasia 2023;41

Activation of the cGAS-STING innate immune response in cells with deficient mitochondrial topoisomerase TOP1MT

Al Khatib I, Deng J, Lei Y, Torres-Odio S, Rojas G, Newman L, Chung B, Symes A, Zhang H, Huang S, Pommier Y, Khan A, Shadel G, West A, Gibson W, Shutt T
Human Molecular Genetics 2023;32(15):2422-2440

Replication-associated formation and repair of human topoisomerase IIIα cleavage complexes

Saha L, Saha S, Yang X, Huang S, Sun Y, Jo U, Pommier Y
Nature Communications 2023;14(1)

A pesticide and iPSC dopaminergic neuron screen identifies and classifies Parkinson-relevant pesticides

Paul K, Krolewski R, Lucumi Moreno E, Blank J, Holton K, Ahfeldt T, Furlong M, Yu Y, Cockburn M, Thompson L, Kreymerman A, Ricci-Blair E, Li Y, Patel H, Lee R, Bronstein J, Rubin L, Khurana V, Ritz B
Nature Communications 2023;14(1)

Mitochondrial uncouplers induce proton leak by activating AAC and UCP1

Bertholet A, Natale A, Bisignano P, Suzuki J, Fedorenko A, Hamilton J, Brustovetsky T, Kazak L, Garrity R, Chouchani E, Brustovetsky N, Grabe M, Kirichok Y
Nature 2022;606(7912):180-187

Top3α is the replicative topoisomerase in mitochondrial DNA replication

Hangas A, Kekäläinen N, Potter A, Michell C, Aho K, Rutanen C, Spelbrink J, Pohjoismäki J, Goffart S
Nucleic Acids Research 2022;50(15):8733-8748

GDAP1 loss of function inhibits the mitochondrial pyruvate dehydrogenase complex by altering the actin cytoskeleton

Wolf C, Pouya A, Bitar S, Pfeiffer A, Bueno D, Rojas-Charry L, Arndt S, Gomez-Zepeda D, Tenzer S, Bello F, Vianello C, Ritz S, Schwirz J, Dobrindt K, Peitz M, Hanschmann E, Mencke P, Boussaad I, Silies M, Brüstle O, Giacomello M, Krüger R, Methner A
Communications Biology 2022;5(1)

Functional characterization of two variants of mitochondrial topoisomerase TOP1MT that impact regulation of the mitochondrial genome

Al Khatib I, Deng J, Symes A, Kerr M, Zhang H, Huang S, Pommier Y, Khan A, Shutt T
Journal of Biological Chemistry 2022;298(10):102420

Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells

Aass K, Mjelle R, Kastnes M, Tryggestad S, van den Brink L, Aass Roseth I, Westhrin M, Zahoor M, Moen S, Vikene Nedal T, Buene G, Misund K, Sponaas A, Ma Q, Sundan A, Groen R, Slørdahl T, Waage A, Standal T
iScience 2022;25(1):103605

A novel mechanism for NLRP3 inflammasome activation

Zhang T, Zhao J, Liu T, Cheng W, Wang Y, Ding S, Wang R
Metabolism Open 2022;13

Extracellular ATP is increased by release of ATP-loaded microparticles triggered by nutrient deprivation

Vultaggio-Poma V, Falzoni S, Chiozzi P, Sarti A, Adinolfi E, Giuliani A, Sánchez-Melgar A, Boldrini P, Zanoni M, Tesei A, Pinton P, Di Virgilio F
Theranostics 2022;12(2):859-874

Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation

Gonzalez Pisfil M, Nadelson I, Bergner B, Rottmeier S, Thomae A, Dietzel S
Scientific Reports 2022;12(1)

FBP2—A New Player in Regulation of Motility of Mitochondria and Stability of Microtubules in Cardiomyocytes

Pietras Ł, Stefanik E, Rakus D, Gizak A
Cells 2022;11(10):1710

Self‐assembly of multi‐component mitochondrial nucleoids via phase separation

Feric M, Demarest T, Tian J, Croteau D, Bohr V, Misteli T
The EMBO Journal 2021;40(6)

ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly

Arguello T, Peralta S, Antonicka H, Gaidosh G, Diaz F, Tu Y, Garcia S, Shiekhattar R, Barrientos A, Moraes C
Cell Reports 2021;37(12):110139

NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation

Simpson C, Tokito M, Uppala R, Sarkar M, Gudjonsson J, Holzbaur E
Cell Reports 2021;34(5):108689

The Tumor Necrosis Factor Alpha and Interleukin 6 Auto-paracrine Signaling Loop Controls Mycobacterium avium Infection via Induction of IRF1/IRG1 in Human Primary Macrophages

Gidon A, Louet C, Røst L, Bruheim P, Flo T, Stallings C
mBio 2021;12(5)

PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B

Bock T, Türk C, Aravamudhan S, Keufgens L, Bloch W, Rozsivalova D, Romanello V, Nogara L, Blaauw B, Trifunovic A, Braun T, Krüger M
Nature Communications 2021;12(1)

Characterization of a novel variant in the HR1 domain of MFN2 in a patient with ataxia, optic atrophy and sensorineural hearing loss

Sharma G, Sabouny R, Joel M, Martens K, Martino D, de Koning A, Pfeffer G, Shutt T
F1000Research 2021;10

A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro

Keller F, Bruch R, Schneider R, Meier-Hubberten J, Hafner M, Rudolf R
Cells 2020;9(8):1900

Intact vitamin A transport is critical for cold-mediated adipose tissue browning and thermogenesis

Fenzl A, Kulterer O, Spirk K, Mitulović G, Marculescu R, Bilban M, Baumgartner-Parzer S, Kautzky-Willer A, Kenner L, Plutzky J, Quadro L, Kiefer F
Molecular Metabolism 2020;42

USP30 sets a trigger threshold for PINK1–PARKIN amplification of mitochondrial ubiquitylation

Rusilowicz-Jones E, Jardine J, Kallinos A, Pinto-Fernandez A, Guenther F, Giurrandino M, Barone F, McCarron K, Burke C, Murad A, Martinez A, Marcassa E, Gersch M, Buckmelter A, Kayser-Bricker K, Lamoliatte F, Gajbhiye A, Davis S, Scott H, Murphy E, England K, Mortiboys H, Komander D, Trost M, Kessler B, Ioannidis S, Ahlijanian M, Urbé S, Clague M
Life Science Alliance 2020;3(8):e202000768

A Splice Intervention Therapy for Autosomal Recessive Juvenile Parkinson’s Disease Arising from Parkin Mutations

Li D, Aung-Htut M, Ham K, Fletcher S, Wilton S
International Journal of Molecular Sciences 2020;21(19):7282

Expression of Fbp2, a Newly Discovered Constituent of Memory Formation Mechanisms, Is Regulated by Astrocyte–Neuron Crosstalk

Hajka D, Duda P, Wójcicka O, Drulis-Fajdasz D, Rakus D, Gizak A
International Journal of Molecular Sciences 2020;21(18):6903

Functional Consequences of PDK4 Deficiency in Doberman Pinscher Fibroblasts

Bolfer L, Estrada A, Larkin C, Conlon T, Lourenco F, Taggart K, Suzuki-Hatano S, Pacak C
Scientific Reports 2020;10(1)

SS-31 Peptide Reverses the Mitochondrial Fragmentation Present in Fibroblasts From Patients With DCMA, a Mitochondrial Cardiomyopathy

Machiraju P, Wang X, Sabouny R, Huang J, Zhao T, Iqbal F, King M, Prasher D, Lodha A, Jimenez-Tellez N, Ravandi A, Argiropoulos B, Sinasac D, Khan A, Shutt T, Greenway S
Frontiers in Cardiovascular Medicine 2019;6

Increased mtDNA Abundance and Improved Function in Human Barth Syndrome Patient Fibroblasts Following AAV-TAZ Gene Delivery

Suzuki-Hatano S, Sriramvenugopal M, Ramanathan M, Soustek M, Byrne B, Cade W, Kang P, Pacak C
International Journal of Molecular Sciences 2019;20(14):3416

The zebrafish orthologue of the human hepatocerebral disease geneMPV17plays pleiotropic roles in mitochondria

Martorano L, Peron M, Laquatra C, Lidron E, Facchinello N, Meneghetti G, Tiso N, Rasola A, Ghezzi D, Argenton F
Disease Models & Mechanisms 2019

Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2

Hangas A, Aasumets K, Kekäläinen N, Paloheinä M, Pohjoismäki J, Gerhold J, Goffart S
Nucleic Acids Research 2018;46(18):9625-9636

The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells

Radif Y, Ndiaye H, Kalantzi V, Jacobs R, Hall A, Minogue S, Waugh M
Molecular and Cellular Biochemistry 2018;448(1-2):275-286

PARL partitions the lipid transfer protein STARD7 between the cytosol and mitochondria

Saita S, Tatsuta T, Lampe P, König T, Ohba Y, Langer T
The EMBO Journal 2018;37(4)

Pgam5 released from damaged mitochondria induces mitochondrial biogenesis via Wnt signaling

Bernkopf D, Jalal K, Brückner M, Knaup K, Gentzel M, Schambony A, Behrens J
Journal of Cell Biology 2018;217(4):1383-1394

Dual role of USP 30 in controlling basal pexophagy and mitophagy

Marcassa E, Kallinos A, Jardine J, Rusilowicz‐Jones E, Martinez A, Kuehl S, Islinger M, Clague M, Urbé S
EMBO reports 2018;19(7)

p62 is linked to mitophagy in oleic acid-induced adipogenesis in human adipose-derived stromal cells

Zeng R, Fang Y, Zhang Y, Bai S
Lipids in Health and Disease 2018;17(1)

Intracellular localization of polymyxins in human alveolar epithelial cells

Ahmed M, Velkov T, Zhou Q, Fulcher A, Callaghan J, Zhou F, Chan K, Azad M, Li J
Journal of Antimicrobial Chemotherapy 2018

PDK4 Deficiency Induces Intrinsic Apoptosis in Response to Starvation in Fibroblasts from Doberman Pinschers with Dilated Cardiomyopathy

Taggart K, Estrada A, Thompson P, Lourenco F, Kirmani S, Suzuki-Hatano S, Pacak C
BioResearch Open Access 2017;6(1):182-191

New protein–protein interactions of mitochondrial connexin 43 in mouse heart

Denuc A, Núñez E, Calvo E, Loureiro M, Miro‐Casas E, Guarás A, Vázquez J, Garcia‐Dorado D
Journal of Cellular and Molecular Medicine 2016;20(5):794-803

APOOL Is a Cardiolipin-Binding Constituent of the Mitofilin/MINOS Protein Complex Determining Cristae Morphology in Mammalian Mitochondria

Cobine P, Weber T, Koob S, Heide H, Wittig I, Head B, van der Bliek A, Brandt U, Mittelbronn M, Reichert A
PLoS ONE 2013;8(5):e63683

APOOL Is a Cardiolipin-Binding Constituent of the Mitofilin/MINOS Protein Complex Determining Cristae Morphology in Mammalian Mitochondria

Weber T, Koob S, Heide H, Wittig I, Head B, van der Bliek A, Brandt U, Mittelbronn M, Reichert A, Cobine P
PLoS ONE 2013 May;8(5)

Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Sample type: Human