Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [8]
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- Product number
- PA5-38027 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAMBI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A recommended positive control is mouse lung tissue lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice.
Adipose-specific BMP and activin membrane-bound inhibitor (BAMBI) deletion promotes adipogenesis by accelerating ROS production.
BAMBI and CHGA in Prion Diseases: Neuropathological Assessment and Potential Role as Disease Biomarkers.
Upregulated microRNA-106a Promotes Porcine Preadipocyte Proliferation and Differentiation by Targeting Different Genes.
Senataxin Mutation Reveals How R-Loops Promote Transcription by Blocking DNA Methylation at Gene Promoters.
Wooten JS, Poole KE, Harris MP, Guilford BL, Schaller ML, Umbaugh D, Seija A
Current research in physiology 2022;5:63-72
Current research in physiology 2022;5:63-72
Adipose-specific BMP and activin membrane-bound inhibitor (BAMBI) deletion promotes adipogenesis by accelerating ROS production.
Chen X, Zhao C, Xu Y, Huang K, Wang Y, Wang X, Zhou X, Pang W, Yang G, Yu T
The Journal of biological chemistry 2021 Jan-Jun;296:100037
The Journal of biological chemistry 2021 Jan-Jun;296:100037
BAMBI and CHGA in Prion Diseases: Neuropathological Assessment and Potential Role as Disease Biomarkers.
López-Pérez Ó, Bernal-Martín M, Hernaiz A, Llorens F, Betancor M, Otero A, Toivonen JM, Zaragoza P, Zerr I, Badiola JJ, Bolea R, Martín-Burriel I
Biomolecules 2020 May 2;10(5)
Biomolecules 2020 May 2;10(5)
Upregulated microRNA-106a Promotes Porcine Preadipocyte Proliferation and Differentiation by Targeting Different Genes.
Huang K, Shi X, Wang J, Yao Y, Peng Y, Chen X, Li X, Yang G
Genes 2019 Oct 14;10(10)
Genes 2019 Oct 14;10(10)
Senataxin Mutation Reveals How R-Loops Promote Transcription by Blocking DNA Methylation at Gene Promoters.
Grunseich C, Wang IX, Watts JA, Burdick JT, Guber RD, Zhu Z, Bruzel A, Lanman T, Chen K, Schindler AB, Edwards N, Ray-Chaudhury A, Yao J, Lehky T, Piszczek G, Crain B, Fischbeck KH, Cheung VG
Molecular cell 2018 Feb 1;69(3):426-437.e7
Molecular cell 2018 Feb 1;69(3):426-437.e7
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of BAMBI in mouse lung tissue lysate using a BAMBI polyclonal antibody (Product # PA5-38027) at a dilution of 1 µg/mL in (A) the absence and (B) the presence of a blocking peptide.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of BAMBI in mouse lung tissue lysate with BAMBI Polyclonal Antibody (Product # PA5-38027) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Supportive validation
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- Experimental details
- Immunofluorescent analysis of BAMBI in human lung tissue using a BAMBI polyclonal antibody (Product # PA5-38027) at a dilution of 20 µg/mL.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescence of BAMBI in human lung tissue with BAMBI Polyclonal Antibody (Product # PA5-38027) at 20 µg/mL.
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- Immunohistochemistry of BAMBI in human lung tissue with BAMBI Polyclonal Antibody (Product # PA5-38027) at 5 µg/mL.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Invitrogen Antibodies (provider)
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- Figure 7 Western blot quantification of BAMBI and CHGA proteins in the CNS of scrapie-infected sheep. ( A ) Membranes displaying the specificity of antibodies against BAMBI and CHGA proteins in the ovine thalamus (T), medulla oblongata (Mo), frontal cortex (Fc) and cerebellum (Cbl) of control and scrapie-infected sheep detected using Western blot. Each protein was cropped and grouped from different parts of the same gel or from different gels. Distinctive bands of ~30 kDa and ~50 kDa confirmed the specificity of the antibodies used against BAMBI and CHGA, respectively. ( B ) Western blot quantification of BAMBI in T and Mo and CHGA in Fc, Cbl and Mo. Density of immunoreactive bands was normalized for beta-Actin density band and is reported as arbitrary units. Data are expressed as means +- standard deviation. Quantification of density of bands did not reveal significant changes in any of the analysed areas, although CHGA displayed a trend to upregulation in scrapie animals (black bars) in Fc ( p = 0.097). Differences between groups were determined using the Student's t -test (# p < 0.1).
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- Invitrogen Antibodies (provider)
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- Figure 1 Generation of adipose-specific BAMBI-knockout mice. A , schematic of the Cre-loxP system used to construct the adipose-specific BAMBI AKO mice model. B , gel electrophoresis analysis of genotype identification of BAMBI AKO mice. C , western blot analysis of BAMBI protein expression in adipose tissue (ingWAT, epiWAT) of 20-week-old BAMBI Flox and BAMBI AKO mice and quantification, with beta-Tubulin as a control. D , body weight and ( E ) food intake statistics of BAMBI Flox and AKO mice fed with HFD diet for 14 weeks (n = 12). F , comparison of body shape and ( G ) weight of mice after 14 weeks of HFD feeding (n = 10). H - L , morphological comparison and weight statistics of the liver, iWAT, eWAT, and BAT after sacrifice of mice (n = 10). M - P , the serum of mice was collected to detect the statistics of TG, TC, HDL, and LDL (n = 8). Data are represented as the mean +- SD. Significance was determined by t -test analysis, * p < 0.05, ** p < 0.001.
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- Figure 2 Adipose-specific BAMBI AKO mice have increased fat deposition and reduced heat production with high-fat diet. A representative image of H&E-stained sections of iWAT tissues from BAMBI Flox and BAMBI AKO mice with HFD diet, scale bar: 50 mum. B , adipocyte area statistics of iWAT from HFD-fed BAMBI Flox and AKO mice (n = 10). C , comparison of mRNA expression levels of Ppargamma, C/ebpbeta, Ap2 in iWAT of BAMBI-Flox and AKO mice, beta-actin as a correction (n = 7), scale bar: 50 mum. D , western blot analysis of PPARgamma, AP2, ATGL, HSL, and BAMBI expression in iWAT of BAMBI Flox and AKO mice and quantification, with beta-Tubulin as a control (n = 4). E , representative image of H&E-stained sections of BAT tissues from BAMBI Flox and AKO mice with HFD diet. Scale bar: 50 mum. F , adipocyte area statistics of BAT from HFD-fed BAMBI Flox and AKO mice (n = 9). G , comparison of mRNA expression levels of Ppargamma, C/ebpbeta, Ap2, Ucp1, Prdm16, and Pgc1alpha in BAT of BAMBI Flox and AKO mice, with beta-actin as a control (n = 6). H , western blot analysis of UCP1, PGC1alpha, PPARgamma, aP2, and BAMBI expression in BAT of BAMBI Flox and AKO mice and quantification, with beta-Tubulin as a control (n = 4). I - K , metabolism studies of control and BAMBI AKO mice fed an HFD: oxygen consumption ( I ), respiratory exchange ratio (RER), ( J ) and heat production ( K ) (n = 4). Data are represented as the mean +- S.D. Significance was determined by t -test analysis, * p < 0.05,
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- Figure 4 Knockout of BAMBI enhances the adipogenesis of adipocytes. A , the RT-qPCR results of adipogenesis-, lipolysis-, inflammation-, and thermogenesis-related genes of in adipocyte isolated from iWAT and BAT, with beta-actin as a control (n = 5). B , western blot analysis of PPARgamma, aP2, GLUT4, HSL, p-ATK, AKT, and BAMBI expression in adipocyte isolated from iWAT and BAT of BAMBI Flox and AKO mice and quantification, with beta-Tubulin as a control (n = 3). C , quantitative results of Oil Red O staining and quantification in iWAT and BAT adipocytes of BAMBI Flox and AKO mice (n = 5), scale bar: 200 mum. D , bodipy staining in iWAT and BAT adipocyte of BAMBI-Flox and AKO mice, DAPI: nucleus, Scale bar: 200 mum. Data are represented as the mean +- SD. Significance was determined by t -test analysis, * p < 0.05, ** p < 0.001. RT-qPCR, real-time quantitative PCR.
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- Fig. 3 Biomarkers of hepatic inflammation and TGFbeta1 and BAMBI protein levels. mRNA expression of markers for inflammatory signaling and proinflammatory cytokines ( A ); protein levels for NF-kappaB p65 ( B ), pNF-kappaB p65 ( C ), pNF-kappaB p65:NF-kappaB p65 ratio ( D ); IL-1beta ( E ), IL-6 ( F ), and TNFalpha ( G ); mRNA expression of TGFbeta1 and BAMBI ( H ); protein levels of TGFbeta1 ( I ) and BAMBI ( J ); and representative Western blots ( K ). Data are presented as mean +- SEM of n = 12 per group for each measured variable. a Significantly different than ND ( p < 0.05), b significantly different than HF ( p < 0.05), and c significantly different than WL ( p < 0.05). Fig. 3
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- Figure 3 miR-106a can target cyclin-dependent kinase inhibitor 1A (p21) instead of BAMBI in the proliferative phase. ( A ) The target site of miR-106a within the porcine p21 mRNA 3'UTR and mutational site of p21 3'UTR; ( B ) a dual-luciferase assay was performed by com-transfection of the miR-106a agomir and wild-type vectors or mutant vectors; relative luciferase activity was represented by Renilla luciferase/firefly luciferase (RLUC/FLUC); ( C ) the relative p21 mRNA expression levels after treatment with the miR-106a agomir; ( D ) western blot analysis of p21 and BAMBI protein expression after treatment with the miR-106a agomir; ( E ) the quantification of p21 and BAMBI protein expression levels. Data are representative of the mean +- SEM of three independent experiments. * p < 0.05, ** p < 0.01.
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- Figure 5 miR-106a can target BMP and activin membrane-bound inhibitor (BAMBI) instead of cyclin-dependent kinase inhibitor 1A ( p21) during adipogenic differentiation. ( A ) The target site of miR-106a within the porcine BAMBI mRNA 3'UTR and mutational site of BAMBI 3'UTR; ( B ) the dual-luciferase assay was performed by com-transfection of the miR-106a agomir and wild-type vectors or mutant vectors. Relative luciferase activity was represented by Renilla luciferase/firefly luciferase (RLUC/FLUC); ( C ) the relative BAMBI mRNA expression levels after treatment with an miR-106a agomir; ( D ) western blot analysis of p21 and BAMBI protein expression after treatment with an miR-106a agomir; ( E ) the quantification of p21 and BAMBI protein expression levels. Data are representative of the mean +- standard deviation of three independent experiments. * p < 0.05; ** p < 0.01.