Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF2045 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Galectin-9 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Circulating levels of PD-L1 and Galectin-9 are associated with patient survival in surgically treated Hepatocellular Carcinoma independent of their intra-tumoral expression levels.
Aspergillus fumigatus enhances human NK cell activity by regulating M1 macrophage polarization.
Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen.
Tim-3 Is Upregulated in NK Cells during Early Pregnancy and Inhibits NK Cytotoxicity toward Trophoblast in Galectin-9 Dependent Pathway.
Galectin-1, -3 and -9 Expression and Clinical Significance in Squamous Cervical Cancer.
Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.
Galectin expression profiling identifies galectin-1 and Galectin-9Δ5 as prognostic factors in stage I/II non-small cell lung cancer.
Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferon.
TIM-3 does not act as a receptor for galectin-9.
Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization.
Upregulation of the Tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection.
The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
Sideras K, de Man RA, Harrington SM, Polak WG, Zhou G, Schutz HM, Pedroza-Gonzalez A, Biermann K, Mancham S, Hansen BE, Bart Takkenberg R, van Vuuren AJ, Pan Q, Ijzermans JNM, Sleijfer S, Sprengers D, Dong H, Kwekkeboom J, Bruno MJ
Scientific reports 2019 Jul 23;9(1):10677
Scientific reports 2019 Jul 23;9(1):10677
Aspergillus fumigatus enhances human NK cell activity by regulating M1 macrophage polarization.
Zhang X, He D, Gao S, Wei Y, Wang L
Molecular medicine reports 2019 Aug;20(2):1241-1249
Molecular medicine reports 2019 Aug;20(2):1241-1249
Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen.
Schroeder JT, Bieneman AP
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 1;199(3):855-865
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 1;199(3):855-865
Tim-3 Is Upregulated in NK Cells during Early Pregnancy and Inhibits NK Cytotoxicity toward Trophoblast in Galectin-9 Dependent Pathway.
Sun J, Yang M, Ban Y, Gao W, Song B, Wang Y, Zhang Y, Shao Q, Kong B, Qu X
PloS one 2016;11(1):e0147186
PloS one 2016;11(1):e0147186
Galectin-1, -3 and -9 Expression and Clinical Significance in Squamous Cervical Cancer.
Punt S, Thijssen VL, Vrolijk J, de Kroon CD, Gorter A, Jordanova ES
PloS one 2015;10(6):e0129119
PloS one 2015;10(6):e0129119
Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.
Leskela S, Serrano A, de la Fuente H, Rodríguez-Muñoz A, Ramos-Levi A, Sampedro-Nuñez M, Sánchez-Madrid F, González-Amaro R, Marazuela M
PloS one 2015;10(4):e0123938
PloS one 2015;10(4):e0123938
Galectin expression profiling identifies galectin-1 and Galectin-9Δ5 as prognostic factors in stage I/II non-small cell lung cancer.
Schulkens IA, Heusschen R, van den Boogaart V, van Suylen RJ, Dingemans AM, Griffioen AW, Thijssen VL
PloS one 2014;9(9):e107988
PloS one 2014;9(9):e107988
Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferon.
McSharry BP, Forbes SK, Cao JZ, Avdic S, Machala EA, Gottlieb DJ, Abendroth A, Slobedman B
Journal of virology 2014 Sep;88(18):10990-4
Journal of virology 2014 Sep;88(18):10990-4
TIM-3 does not act as a receptor for galectin-9.
Leitner J, Rieger A, Pickl WF, Zlabinger G, Grabmeier-Pfistershammer K, Steinberger P
PLoS pathogens 2013 Mar;9(3):e1003253
PLoS pathogens 2013 Mar;9(3):e1003253
Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization.
Baitsch L, Legat A, Barba L, Fuertes Marraco SA, Rivals JP, Baumgaertner P, Christiansen-Jucht C, Bouzourene H, Rimoldi D, Pircher H, Rufer N, Matter M, Michielin O, Speiser DE
PloS one 2012;7(2):e30852
PloS one 2012;7(2):e30852
Upregulation of the Tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.
Nebbia G, Peppa D, Schurich A, Khanna P, Singh HD, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, ChinAleong J, Kennedy P, Maini MK
PloS one 2012;7(10):e47648
PloS one 2012;7(10):e47648
A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection.
Mengshol JA, Golden-Mason L, Arikawa T, Smith M, Niki T, McWilliams R, Randall JA, McMahan R, Zimmerman MA, Rangachari M, Dobrinskikh E, Busson P, Polyak SJ, Hirashima M, Rosen HR
PloS one 2010 Mar 4;5(3):e9504
PloS one 2010 Mar 4;5(3):e9504
The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
Thijssen VL, Hulsmans S, Griffioen AW
The American journal of pathology 2008 Feb;172(2):545-53
The American journal of pathology 2008 Feb;172(2):545-53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Galectin-9 by Western Blot. Western blot shows conditioned media from HT-29 human colon adenocarcinoma cell line, human peripheral blood lymphocytes (PBL), and human dendritic cells untreated (-) or treated (+) with 10 µg/mL PHA for 6 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Galectin-9 at approximately 36 and 40 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Galectin-9 by Western Blot. Detection of Human Galectin-9 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Galectin-9 in Human Colon. Galectin-9 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.