PA5-26030

antibody from Invitrogen Antibodies

Targeting: MAPK8IP1 IB1, JIP-1, JIP1, PRKM8IP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-26030

Provider

Invitrogen Antibodies

Product name

JIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.45 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of JIP1 in various lysates. Samples were incubated with JIP1 polyclonal antibody (Product # PA5-26030) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: human brain lysate; Lane 2: MCF-7 whole cell lysate. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using a JIP1 polyclonal antibody (Product # PA5-26030) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of JIP1 in MCF-7 cells. Samples were incubated in JIP1 polyclonal antibody (Product # PA5-26030) followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of JIP1 in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with JIP1 polyclonal antibody (Product # PA5-26030) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of JIP1 in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with JIP1 polyclonal antibody (Product # PA5-26030) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of MCF-7 cells using a JIP1 polyclonal antibody (Product # PA5-26030) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of JIP1 in MCF-7 cells (bottom histogram). Samples were incubated with JIP1 polyclonal antibody (Product # PA5-26030) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).