15678-1-AP

antibody from Proteintech Group

Targeting: CDC45 CDC45L, CDC45L2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

15678-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15678-1-AP, RRID:AB_10642704

Product name

CDC45L antibody

Antibody type

Polyclonal

Description

CDC45L antibody (Cat. #15678-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Design and synthesis of novel 1,2,4-triazolobenzene sulfonamide derivatives as selective CDK1 inhibitors with potent in vivo anticancer efficacy.

Wu L, Wang N, Lu Y, Wang Y, Ma L, Lou G, Wang N, Jiang A, Ding L, He S, Zhang B
European journal of medicinal chemistry 2026 Jun 5;310:118799

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Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage.

Zhang J, Wang Y, Cao S, Xie SM, Liu B, Li Y, Hou Y, Meng X, Ruan M, Bu D, Kang J, Li R, Lou L, Wang J, Xing L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(35):e09307

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MCM5 UFMylation regulates replication origin firing and fork progression.

Li Z, Wu X, Liu L, Rao S, Liao Y, Liu M, Peng B, Zhang Q, Xia Y, Zhai Y, Takeda S, Xu X
The EMBO journal 2025 Nov;44(21):6019-6050

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Dynamic regulation of origin firing factors links CDK activity to dormant origin activation.

Hossain MS, Sansam CG, Wittig KA, Noble TD, Boyd KA, Sansam CL
bioRxiv : the preprint server for biology 2025 Jun 11;

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IGF2BP2 promotes glycolysis and hepatocellular carcinoma stemness by stabilizing CDC45 mRNA via m6A modification.

Wu T, Liao L, Wu T, Chen S, Yi Q, Xu M
Cell cycle (Georgetown, Tex.) 2023 Oct;22(20):2245-2263

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LSD1 is required for euchromatic origin firing and replication timing.

Wang Y, Huang Y, Cheng E, Liu X, Zhang Y, Yang J, Young JTF, Brown GW, Yang X, Shang Y
Signal transduction and targeted therapy 2022 Apr 13;7(1):102

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The CRL4DTL E3 ligase induces degradation of the DNA replication initiation factor TICRR/TRESLIN specifically during S phase.

Wittig KA, Sansam CG, Noble TD, Goins D, Sansam CL
Nucleic acids research 2021 Oct 11;49(18):10507-10523

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Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.

Xie W, Wang B, Wang X, Hou D, Su H, Huang H
Annals of translational medicine 2020 Apr;8(7):478

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The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Fan L, Cao X, Yan H, Wang Q, Tian X, Zhang L, He X, Borjihan G, Morigen
Oncotarget 2017 Jul 18;8(29):47250-47268

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Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells.

Kliszczak M, Sedlackova H, Pitchai GP, Streicher WW, Krejci L, Hickson ID
Oncotarget 2015 Dec 1;6(38):40464-79

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 15678-1-AP(CDC45L antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using CDC45L antibody 15678-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line