Explore
Validate
Learn
Western blot
ELISA
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C675489 - Provider product page
- Provider
- LSBio
- Product name
- CDC45 Antibody LS-C675489
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All Lanes:CDC45 antibody at 0.3 ug/ml Lane 1: K562 whole cell lysate Lane 2: 293T whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG-2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 66,61,69 kDa Observed band size: 66,58 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating CDC45 in K562 whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ K562 whole cell lysate(500ug) Lane 3: K562 whole cell lysate (20ug)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating CDC45 in K562 whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ K562 whole cell lysate(500ug) Lane 3: K562 whole cell lysate (20ug)
